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Rebecca M. Hamner Advisor: Wilson Freshwater

The use of DNA markers to identify and explore the genetic diversity of lionfishes Pterois miles and Pterois volitans. Rebecca M. Hamner Advisor: Wilson Freshwater. P. miles P. volitans Mixed Invasive Pterois sp. Current Data

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Rebecca M. Hamner Advisor: Wilson Freshwater

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  1. The use of DNA markers to identify and explore the genetic diversity of lionfishes Pterois miles and Pterois volitans Rebecca M. Hamner Advisor: Wilson Freshwater

  2. P. miles P. volitans Mixed Invasive Pterois sp.

  3. Current Data • Disagreement between mitochondrial DNA and meristic identities • 14 nuclear DNA loci lack species specific variation for P. miles and P. volitans Act-2 AldB Cam-3 CK CKA7 ChymB-6 ITS LDH LDHA6 Mlc-3 Ops-1 RP1 RP2 SSU

  4. Objective I • Investigate hybridization using Randomly Amplified Polymorphic DNAs (RAPDs) to develop a nuclear DNA marker to distinguish between Pterois miles and Pterois volitans

  5. MethodsRandomly Amplified Polymorphic DNAs (RAPDs) 1. Polymerase Chain Reaction 5’ 3’ 4 1 2 3 Template DNA Primer sites Copied DNA fragments

  6. 2. Gel Electrophoresis A Size marker ? B longest fragments shortest fragments

  7. Objective II • Examine the genetic diversity of North Carolina lionfish to determine their source population ?

  8. ? Methods NC lionfish PCR & Sequence Reaction ABI 3100 Genetic Analyzer Phylogenetic tree or Cluster analysis

  9. Questions? Leo (NC lionfish) at CMS

  10. Mer = m Mito = v Nuc = v Mer = v Mito = m Nuc = m Mer = m Mito = v Nuc = m Mer = v Mito = m Nuc = v Meristic and DNA disagreement Meristic characters not fixed for P. miles and P. volitans Nuclear and mitochondrial DNA disagreement -Hybridization -Incomplete lineage sorting Mer = meristic counts Mito = mitochondrial DNA Nuc = nuclear DNA

  11. 2. Gel Electrophoresis Partis, L. and R. J. Wells. 1996. Identification of fish species using random amplified polymorphic DNA (RAPD). Molecular and Cellular Probes, 10: 435-441.

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