Conservation of Key Residues in Dynamin Proteins

1. Leu566His Gly533Cys VI RR G WL T I NN I S LM K G G S K E Y WFVL T A E S L S W Y K DEEE K E KK Y MLPL D N L K I R D V E K G FM S DNM2 H.sapiens VI RR G WL T I NN I S LM K G G S K E Y WFVL T A E S L S W Y K DEEE K E KK Y MLPL D N L K I R D V E K G FM S DNM2 M. musculus DNM3 H. sapiens DNM2R. norvegicus VI RR G WL T I NN I S LM K G G S K E Y WFVL T A E S L S W Y K DEEE K E KK Y MLPL D N L K I R D V E K G FM S DNM1 H. sapiens VI RK G WL T I NN I G IM K G G S K E Y WFVL T A E N L S W Y K DDEE K E KK Y ML S V D N L K L R D V E K G FM S Dynamin D. rerio VI RK G WL T I SN I G IM K G G S K GY WFVL T A E S L S W Y K DDEE K E KK Y MLPL D N L K V R D V E K S FM S Dynamin D. melanogaster VI RK GH MVI QN L G IM K G G S R P Y WFVL TS E S I S W Y K DEDE K E KK FMLPL D G L K L R D I E QG FM S VI RR G WL T I N - I S IM K G G S K D Y WFVL T A E S L S W Y K DEEE K E KK FMLPL D N L K L R D V E K S FM S MLPL D G V K L K D I E GG FM S VI RK G WL S L SN V S FV R G – S K D N WFVLM S D S L S W Y K DDEE K E KK Y Dynamin C. elegans E-Figure 1. Alignment of DNM2 orthologs and paralogs spanning PH from amino acids 516-577. Residues 533 and 566 mutated by the identified nucleotide changes are shown within boxes and are highly conserved. Small and hydrophobic amino acids are depicted inred; acidic amino acids in blue; basic amino acids in magenta; and amino acids with hydroxyl, amine and basic chains in green.