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slide4

Explants from folded brown leaves were superior in induction of callus. Half strength MS medium fortified with 0.88 microM of benzyiadenine (BA), 0.9 microM of 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.46 microM of kinetin (Kn) at pH 5.5 was most effective for callus induction.

Transfer of callus to medium with 0.54 microM of NAA in place of 2,4-D induced higher number of shoots. Subsequent cultures displayed enhanced rate of shoot initiation and multiplication. Transfer of shoots onto half strength MS medium supplemented with 0.54 microM of NAA favoured rooting of shoots.

in vitro process
In vitro process

Leaf disc

Callus formation

Greenhouse established plants

In vitroplantlets

slide7

Segregation of transgenic B. napus on A1 medium containing 50 mg/l of kanamycin

Wt

Transformants

Wt

Transformant

Transformants

back side

back side

12 days

19 days

5 days

22/3/07

micro propagation of mangrove plant
Micro propagation of Mangrove plant

Tissue culture of Antherium

black beauty

Transgenic Vanilla resistant to patogen