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Full title of paper: A comparison study of Agrobacterium -mediated transformation methods for root-specific promoter analysis in soybean Journal name: Plant Cell Reports (PCR) Authors name: Caifeng Li, Haiyan Zhang, Xiurong Wang* and Hong Liao

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Full title of paper: A comparison study of Agrobacterium-mediated transformation methods for root-specific promoter analysis in soybean

Journal name: Plant Cell Reports (PCR)

Authors name: Caifeng Li, Haiyan Zhang, Xiurong Wang* and Hong Liao

Address: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Root Biology Center, South China Agricultural University, Guangzhou 510642, China

E-mail address of correspondence author: xrwang@scau.edu.cn

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LP HP

*

Online Resource 3. Expression analysis of GmPAP21 in soybean transgenic hairy roots under both LP and HP conditions by qRT-PCR.

The relative expression value was calculated by the expression ratio between GmPAP21 and soybean housekeeping gene EF-1a. Data in the figure are the mean of biological replicates from three independent hairy root transformation experiments with standard error. Asterisks indicate significant differences between LP and HP treatments (LSD test, P < 0.05).