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Image-Pro Premier Basic Training Course

In this module, we explore automated counting methods in Image-Pro, highlighting their efficiency over manual measurements. Manual counting can be time-consuming and inconsistent, while automated techniques utilize pixel information to segment images and accurately count objects. Learn how to set up counting options, select measurement types, and display/export your data effectively. We'll cover segmentation strategies, including automatic identification of objects based on brightness, and provide insights into adjusting connectivity settings for precise results. Enhance your image analysis skills with this comprehensive training.

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Image-Pro Premier Basic Training Course

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  1. Image-Pro PremierBasic Training Course • Part 5 - Automated Counting

  2. Automated Counting • Manual measurements take time and are difficult to repeat reliably • Objects in images often share intensity characteristics that are different from background or other object • By identifying groups of pixels based on pixel information we can automatically measure objects

  3. Automated Counting • Segment image • Set up counting option • Select measurement types • Count • Display and save/export data

  4. Segmentation • Divide pixels from the image into segments corresponding to: • Objects or one or more class • Background • Image-Pro can automatically identify bright or dark objects • Manual segmentation give more flexibility

  5. Automatically Segmentation • Go to Count/Size ribbon • In Segment section select Bright or Dark • Pixels identified according to the the selection are highlighted on the image

  6. Counting Options • Display Options - Affect look of objects • Segmentation and Count Options - Affect how objects are measured

  7. Connectivity 4 Connect 8 Connect Only pixels sharing a side are touching Any neighbouring pixel can be touching

  8. Clean Borders

  9. Clean Borders • % Surface Area - Partial objects not important, holes probably need to be ignored - Clean borders > None • Morphology - Only whole objects to be measured, holes likely part of the objects - Clean borders > All • Counting - Need to avoid double counting partial objects, 1/2 objects on borders - Clean borders > 2 Adjacent

  10. Measurement Types • Same as manual measurements • Select measurements relevant for object type • Use measurements for filtering

  11. Displaying Data • When objects are counted, measurement table is automatically shown • Use Measurements Histogram to show spread • Use classification to enhance display on Image

  12. Exporting Data • Quick Save for Publication to save image with overlays • Quick Save for Analysis to save image with measurements for review later • Export function in Measurements table to save data to Excel, a text file or for importing into Premier

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