1 / 6

K.E. Taylor et al. ATVB 2005;25:1225-1230

Azide in cCRP is responsible for reduced cell proliferation, change in cell morphology, and increased apoptosis and cell death.

lalasa
Download Presentation

K.E. Taylor et al. ATVB 2005;25:1225-1230

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Azide in cCRP is responsible for reduced cell proliferation, change in cell morphology, and increased apoptosis and cell death HUVECs (A through D) or EA.hy926 cells (E) were incubated with various CRP preparations and azide. Incubations: control (medium alone;-); rCRP (R); aCRP (A); cCRP (C); azide (Az); or rCRP and azide (RAz). A, Phase contrast light microscopy of confluent HUVECs cultured for 2 days. B, Cell counts of HUVECs seeded at low density and cultured for 6 days. D indicates dCRP. C, Annexin V–FITC binding of HUVECs cultured for 2 days. D, PI uptake by HUVECs cultured for 2 days; E, pH of tissue culture supernatants of EA.hy926 cells cultured for 1 day. Data points marked with asterisks were statistically different from the control treatment: **P<0.01; ***P<0.001. K.E. Taylor et al. ATVB 2005;25:1225-1230

  2. Azide in cCRP is responsible for reduced eNOS expression K.E. Taylor et al. ATVB 2005;25:1225-1230

  3. LPS and not CRP or azide induces ICAM-1 expression in HUVECs K.E. Taylor et al. ATVB 2005;25:1225-1230

  4. CRP does not reduce vWF secretion or increase IL-8 and MCP-1 secretion Supernatants were harvested and analyzed for vWF K.E. Taylor et al. ATVB 2005;25:1225-1230 HUVECs were incubated for 24 hours with medium alone (); rCRP (R); aCRP (A); cCRP (C); dialyzed cCRP (D); azide (Az); rCRP and azide (RAz); LPS (0.8 ng/mL); or LPS and azide (LPSAz). Results are expressed as meanSEM of experiments performed in triplicate. Data points marked with aster-isks were statistically different from the control treatment: *P<0.05; **P<0.01; ***P<0.001.

  5. CRP does not reduce vWF secretion or increase IL-8 and MCP-1 secretion Supernatants were harvested and analyzed for MCP-1 K.E. Taylor et al. ATVB 2005;25:1225-1230 HUVECs were incubated for 24 hours with medium alone (); rCRP (R); aCRP (A); cCRP (C); dialyzed cCRP (D); azide (Az); rCRP and azide (RAz); LPS (0.8 ng/mL); or LPS and azide (LPSAz). Results are expressed as meanSEM of experiments performed in triplicate. Data points marked with aster-isks were statistically different from the control treatment: *P<0.05; **P<0.01; ***P<0.001.

  6. CRP does not reduce vWF secretion or increase IL-8 and MCP-1 secretion Supernatants were harvested and analyzed for IL-8 K.E. Taylor et al. ATVB 2005;25:1225-1230 HUVECs were incubated for 24 hours with medium alone (); rCRP (R); aCRP (A); cCRP (C); dialyzed cCRP (D); azide (Az); rCRP and azide (RAz); LPS (0.8 ng/mL); or LPS and azide (LPSAz). Results are expressed as meanSEM of experiments performed in triplicate. Data points marked with aster-isks were statistically different from the control treatment: *P<0.05; **P<0.01; ***P<0.001.

More Related