Welcome to Central Facilities for Research and Development (CFRD )

1. Welcome to Central Facilities for Research and Development(CFRD) Central Facilities for Research and Development

2. About University

3. About University • Osmania University established in 1918. • It is the SEVENTH oldest in the Country. • THIRD oldest in South India. • FIRST in the princely state of Hyderabad. • The establishment of Osmania University symbolizes a renaissance in the Indian Educational System. • Shown a remarkable resilience throughout its existence. • The University is instrumental in propagation of Higher Education to cater to the needs of community in the region over nine decades and has grown into one of the major Universities of India. Central Facilities for Research and Development

4. The University is surrounded by nine National Laboratories, which are recognized by Osmania University as Centers for Advanced Research in the fields of Science and Technology in addition to Arts and Social Science Research Centers. • Osmania University has been encouraging Staff, Scholars and Students in achieving world class research. • Osmania University receives research funds from UGC-SAP, DST-FIST, ICMR, ICAR,CSIR, NBMP, DBT, DAE, AICTE etc. • The funds received from the agencies to procure equipment may be self sufficient but to maintain the procured equipments beyond the project period is becoming very difficult. • Further, there is duplication in procuring equipment as the projects are being sanctioned to individual departments and to individual researchers. Central Facilities for Research and Development

5. About Central Facilities for Research and Development

6. About Central Facilities for Research and Development • In order to streamline the hardships faced by the Departments of University would like to make amendments and desires to conduct interdisciplinary research in all the departments. • The University is also interested in extending its services to outside Research community, Industries and Academic bodies. • Taking into consideration the above facts the University, in pursuit of academic excellence, has established “CENTRAL FACILITIES for RESEARCH & DEVELOPMENT“ to cater to needs of the students, research scholars and faculty . • Constructed with state of art technology. • Providing corporate infrastructure. • Laboratories equipped with expensive scientific equipments. Central Facilities for Research and Development

7. Help Desk e-class room, Scholars computation Lab and Auditorium. • Facilities thrown open for the benefit of the students, research scholars and faculty. • Central Facilities building has become the nerve centre and more attractive to the eager learners and research community. • Entry into the Central Facilities is restricted to only registered users through biometric access system installed at the entrance door. • After completion of registration the end-user will be issued a CF User ID and Biometric image of the end-user is captured to enter into the building to use the services of Central Facilities. • CCTV cameras are installed at designated sensitive places to provide round the clock surveillance . Central Facilities for Research and Development

8. Help Desk • Established to facilitate easy access to the Staff, Research scholars, Students and visitors of the Centre. • New users registration. • Sample receiving and handing over the test result analysis. • Receipt generation for Central Facilities services charges. • Enquiring management system. • Biometric Monitoring Management system. • Central Facilities Telephone Exchange Monitory. Central Facilities for Research and Development

9. UGC and DBT Scientific Instrumentation Labs • UGC Central Instrumentation Lab. • UGC NMR Lab. • UGC Radiation Lab . • DBT Central Instrumentation Lab. • DBT Microbiology Modular Lab. • DBT Bio Chemistry Modular Lab . • DBT Botany Modular Lab. • DBT Genetics Modular Lab • DBT Zoology Modular Lab Central Facilities for Research and Development

10. Scientific Equipment (Procured under UGC XI Plan grants) • NMR 400 MHZ Spectrometer • Confocal Microscope • FTIR Spectrophotometer • UV-VIS Spectrophotometer • HPLC System • AKTA Purifier • Liquid Scintillation Counter • Ultra Centrifuge Beckmaw • LCMS ( courtesy of Department of Bio-chemistry) Central Facilities for Research and Development

11. Scientific Equipment (Procured under UGC XI Plan grants)

12. NMR – 400 MZ • Make: BRUKER BIOSPIN, SWITZERLAND • Model: Avance-III 400 MHz Fourier Transform Digital liquid NMR Spectrometer • Operator console : All the operations relevant to data acquisition are controlled by second computing system called IPSO (intelligent pulse sequence Organizer) housed within the console itself. • Magnet : The magnet generates the magnetic field required to induce NMR transitions. • Emitted signal from sample nuclei relatively weak amplified by HPPR Setup (high performance Preamplifier) • Detected signals received by console with negligible loss

13. NMR – 400 MZ Applications • It is a powerful tool in structure verification of Organic molecules. • It is a powerful technique for characterization of a wide range of natural products, raw materials, intermediates and manufactured items especially used in conjunction with other spectrometric methods. • It has ability to identify major molecular structural features is useful in synthetic routes to help establish the nature of competitive products such as polymers, paints, organic chemicals, and Pharmaceuticals. • It gives proton spectra which is useful to reveal facts in Organic Structure. • It is used to determine the F-19, P-31, N-15 and C-13 nuclei in given compound with spectral reports. • NMR system analysis is helpful in Organic Chemistry, Pharma / Biotech, and Biochemistry Research.

14. CONFOCAL LASER SCANNING MICROSCOPE Make: Leica Microsystems, Germany Model: Leica TCS SP5-II • APPLICATIONS; • Live cell imaging with using fluorescence • emission of the sample technique. • Provides high resolution image with new imaging techniques FRET, FRAP, DAPI, FITC, Dred etc. • To study of Micro-organisms and their action on living tissues. • Regular inspection of Growing cells i.e. Cell Culture Studies. • Allows scientific Study in plant science to determine structure, growth, metabolism and deceases of plants. Central Facilities for Research and Development

15. FTIR Spectrophotometer • MAKE : Bruker, SWITZERLAND. • MODEL : TENSOR27. • FT-IR Spectrometer with 0.4cm-1 Resolution [Apodised], KBr Beamsplitter. • Permanently aligned cube corner Rock Solid interferometer . • Mid-IR, Near- IR, and Far- IR capability. • Easy to exchange QuickLock sample compartment accessories. • Automatic H2O and CO2 compensation. • Advanced DigiTect Detector system . • OPUS Software including spectral search facility. Central Facilities for Research and Development

16. FTIR Spectrophotometer - Applications • Infrared Spectroscopy gives information on the vibrational and rotational modes of motion of a molecule. • The Infrared spectrum of an organic compound provides a unique fingerprint, which is readily distinguished from the absorption patterns of all other compounds; only optical isomers absorb in exactly the same way. Hence FTIR is an important technique for identification and characterization of a substance. • Used for the identification and quantification of organic species. Is capable of ultra trace organic analysis, e.g. for contamination on surfaces. • Identification of functional group and structure elucidation. Identification of drug substance and impurities in a drug sample. • Allows rapid analysis of micro-samples down to the nanogram level in some cases. Study of polymers, study of hydrogen bonding, Ratio of Cis-trans isomers in a mixture. • Allows Contamination detection, Raw material testing, Measurement of layers by specular and diffuse reflection. Central Facilities for Research and Development

17. ULTRA CENTRIFUGE • Make : Thermo Fischer scientific • Model : SORVALL WX 100 • it is provided with 3 rotors of which one is zonal swinging bucket rotor and the other 2 are vertical fixed angle rotors. • The ultracentrifuge can reach maximum speed of 100,000 rpm. • The system has the temperature control in the range of 00C to 400C. • Fixed Angle rotor T- 8100 with Maximum speed of 100,000 rpm and Max RCF is 802,006 x g with a capacity of 8 tubes of 6.5ml capacity each. Central Facilities for Research and Development

18. Liquid Scintillation Counter • Make: Hydex, Finland. • Model: 300SL counter. • It detects radiation emitted by a sample dissolved in a solvents like Aqualight / Maxlight. • Scintillation Counter is used to detect radiations such as α, β & γ from radio active nucleas. • 520mm (width), 680mm (height), 630mm (depth) flexible Counting system. • Vials are placed in sample tray with matrix format of 8 by 12 totals 96 7ml vials, alternatively 5x8 matrix totals 40 20ml vials also used. • Instrument connected to PC Mikrowin 2000 software, it has easy to use interface with unlimited number of pre stored isotopes and protocols with other analysis features.

19. Liquid Scintillation Counter - Applications • With TDCR technology which is a straight forward method obtaining counting efficiency of samples without any standard source of radioactivity. • Good counting efficiencies, for H-3 >65 %, for C-14 >95%, S-35, for P-32 nearly 100%, P-33, I-125 (beta-emitters). • High Counting efficiency Alpha emitters (Po-210,U-234/U-238,Am-241,Rn-222,Ra-226) > 95%

20. UV-VIS Spectro Photometer • Make : LABINDIA • MODEL : LABINDIA UV-3000+ • Double Beam with Photo silicon Diode Array System • Variable Slit width 0.5 to 5 nm • Automatic 8-Cell Changer • Wavelength range includes 190 to 1100nm • Photometric mode includes Analysis of Transmittance, Absorption, Energy and Concentration analysis. • UV-WIN Software Operational modes . Central Facilities for Research and Development

21. UV-VIS Spectro Photometer - Applications • UV-Vis spectrophotometers have extensive applications in the fields such as Chemicals, Pharmaceutical, Biotechnology, Environmental, Clinical & Food, Beverages, etc. • UV/VIS Spectrophotometers are able to carry out Photometric measurement, Spectrum scans, Kinetic measurements, Qualitative determination and DNA/RNA analysis. • Measuring Absorbance/Transmittance/Concentration values in spectrum form • Can plot the calibration curve with known standards. • Measuring time dependent absorption variance. • Can determine the absorbance spectrum of a sample solution by choosing spectrum mode in the UV-WIN SOFTWARE Central Facilities for Research and Development

22. HPLC – (High Pressure Liquid Chromatography) Central Facilities for Research and Development

23. HPLC • Make : SHIMADZU. • Model : Shimadzu’s Prominence HPLC system. • HPLC system has two pumping systems . • DEGASSER, to remove the gas bubbles. • Automatic sample injector called AUTOSAMPLER. • Non polar columns RP C8 and RP C18. • Column oven with Peltier cooling facility, Fibre optic technology. • Detectors are RID detector, RF detector, and PDA detector . • The software used LC SOLUTION. Central Facilities for Research and Development

24. HPLC - Applications • Reversed phase chromatography has been applied to the purification of biomolecules such as peptides, proteins and oligonucleotides. • Separation and Quantification of the unacetylated and acetylated oligopeptides. • Lipid Analysis provides identification of various classes of lipids like free fatty acids, fatty alcohols, phospholipids, mono-, di-, and triacyl-glycerides, sterols and steroids, and fat soluble vitamins. • Molecules that posses some degree of hydrophobic character, such as Proteins, Peptides, Antibodies, and Nucleic Acids, can be separated by reverse phase chromatography • Phosphopeptides structural characterization and analysis. Central Facilities for Research and Development

25. AKTA – Purifier • Is a preparative mode of • chromatography system based • on ion-exchange principle. • This instrument is supplied by • the GE Health care • The ÄKTA purifier is a fully automated liquid chromatography system designed for method development and research applications • The system offers automated purification and characterization of peptides and nucleic acids. • It finds its wide range of application in vari ous fields of life sciences like in biochemistry, microbiology, biotechnology, botany, zoology etc. Central Facilities for Research and Development

26. E – Class Room Central Facilities for Research and Development

27. E – Class Room • Offer learning through technology • Provides more efficient, effective services in future for complete learning experience. • E-class room provides WEBCASTING Technology • “Online-learning” and “Offline –learning” activities • “Active-learning" and “Passive-learning“ activities • E-Classroom bridges gap between campus locations and off-campus locations . • E-class room accommodates 55 learners with a provision to connect Laptop/Desktop under LAN with live Internet facility. Central Facilities for Research and Development

28. Scholars Computation Lab • Computing laboratory with 40 computers is established with Internet/Intranet connectivity. • Provides software like SPSS, MATLAB, MATHEMETICA, Schrödinger and other software required for research purpose • Users can browse • on-line generals • e-books • University e-repositories (Intranet access ) • OsmaniaUniversity Digital Library Collections • e-class room activities. Central Facilities for Research and Development

29. Osmania University Digital Library • Osmania University has a rich collection of manuscripts, old books which are digitized at the general Library (more than 40,000 books ). • 1000 video/multi media lectures produced at EMMRC (UGC). • Reference collections of OU staff. • Dissertations, Ph.D thesis. • OU Digital Library website (oudl.osmania.ac.in ) is created and maintained at Central Facilities for the benefit of Osmania Students and Research community. Central Facilities for Research and Development

30. Server Room • Server room is the heart of the central facilities as it caters to all the electronic requirements (Network connections, Server, Webcasting Equipment and CCTV network etc) of the building. • The server room is equipped with four rack servers, one storage device , workstations, LAN Network Unit, CCTV Network Unit • Software installed • Windows 2003 server • Red Hat Linux Server • Debian server • Storage Device of 3.6 TB • Administrative software Central Facilities for Research and Development

31. Inhouse Software Developed • Help desk software • Enquiry Management System • Inward and Outward Management System • CFRD Messaging and Attendance Management System • Biometric Software • Osmania University Central Facilities Website (cfrd.osmania.ac.inunder the link central facilities) • Osmania Digital Library Website (oudl.osmania.ac.in) • Development of Online testing software is in progress for the Competitive examination centre. • Any other Intranet/Internet Application Software can be installed as and when required. Central Facilities for Research and Development

32. Thank you Central Facilities for Research and Development