BIOCHEMICAL TESTS. PART ONE. Differentiation of organisms based on their ability to break down complex Macromolecules in to simpler nutritional constituents. Fat. Protein. Starch. Macromolecules are polymers of monomeric subsunits.
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Differentiation of organisms based on their ability to break down complex
Macromolecules in to simpler nutritional constituents
Hydrolysis reactions cleave polymers into monomers by adding water.
And other lipids)
Muscles store sugar as glycogen.
The hydrolysis reaction is the reverse of the dehydration reaction
E. coli B.cereus P.aeruginosa
4 Phenol Red Glucose Broth Tubes
4 Phenol Red Sucrose Broth Tubes
4 Phenol Red Lactose Broth Tubes
E.coli, P.vulgaris, A.faecalis, S.aureus
based on their ability to ferment glucose, lactose and/or sucrose
1% Lactose Phenol Red
1% Sucrose Sodium thiosulfate
Dark color: Hydrogen Sulfide produced