CHE 5480 Summer 2005 5FG

1. CHE 5480 Summer 2005 5FG • Introduction to nanotechnology and supercomputing. • Instructors: Lloyd L. Lee (lle@ou.edu) • Gerald K. Newman (gknewman@ou.edu) • Henry Neeman (hneeman@ou.edu) • Web page: coecs.ou.edu/lllee/www/nanocourse2005.html • Class sponsored by National Science Foundation (CISE/EIA)

2. Instructors

3. What to learn: • Lectures on nanotechnology • Learn high performance computing • Wet labs • Attend nanotechnology meeting

4. Projects: • Chemical and biosensors • Nanobiotechnology • Nanostructures and applications: • =dendrimers, carbon nanotubes, zeolites, aerogels, nanowires, nanoparticles. • Nanofluidics • Nanocomposites (heat management) • Superhydrophobic surfaces—friction/drag reduction • Laboratory-on-a-chip • Homeland security, (others)

5. Textbook: • Eric Drexler, "Engines of Creation" (Anchor Books, 1987) • Downloadable from Foresight.Org: • URL http://www.foresight.org/EOC

7. Web Pagecoecs.ou.edu/lllee/www/nano2005.html

8. Grading: • Homeworks 10% • Midterm Report 20% • Midterm Presentation 10% • Midterm Exam 20% • Final Report 40% • No final written test.

9. Wet Lab:

10. Nanotechnology Meeting:Houston, Texas, July 28, 2005

13. James R. Baker Jr. University of Michigan Professor, Internal Medicine and Bioengineering Chief, Division of Allergy Director, Center for Biologic Nanotechnology Co-Director, Center for Biomedical Engineering Biotechnology, Nanotechnology and Immunology

14. Drug Delivery • Research in the area of autoimmune endocrine disease. He has helped define the basis of the autoimmune response to thyroid auto antigens. Gene Delivery • Work concerning gene transfer; developing a new vector system for gene transfer using synthetic polymers (dendrimers). Anti-microbial research • Work on preventing pathogens from entering the human body. This research project seeks to develop a composite material that will serve as a pathogen avoidance barrier and post-exposure therapeutic agent to be applied in a topical manner to the skin and mucous membranes.

15. Drug Delivery by dendrimers Project called “smart Bombs”: Target cancerous cells and leave the normal intact. • Recognition and diagnosis of cancer • Drug delivery • Location of c cells • Kill by releasing agents

16. Drug Delivery by dendrimers Dendrimers • Known for several applications • Able to enter cells • Little toxicity Focus: • High energy lasers or sound wave energy to trigger the release of the drug out of the dendrimer.

17. Antimicrobial Nanoemulsion • Use of soybean oil emulsified with surfactants. Drops ~400 – 600 nm. • The droplet do not coalesce with themselves . High surface tension make them coalesce with other lipid droplets, killing bacteria. • Safe for external use. Not safe for red cells, or sperm.

18. The droplets fuse with cell membrane of microorganisms resulting in cell lysis. • Very effective in killing: – Bacteria,  – Bacterial spores,  – Enveloped viruses, and – Fungal spores. • They are effective at preventing illness in individuals, when used both before and after exposure to the infective agent. • They could be used:  – Topically,  – As an inhalant.

19. Antimicrobial Nanoemulsion • Left: treated with nanoemulsion, • Right: untreated. • The growth of bacteria colonies has been eliminated by treatment with nanoemulsion.

20. Gene Transfer • G-5 dendrimers of Poly(amidoamine) • The dendrimer is acetyladed to increase solubility. • Fluorescein is incorporated onto the dendrimer for imaging in vivo. • Folic acid is then conjugated as targeting agent. • The final step is to conjugate the therapeutic drug.

21. Gene Transfer • Into cardiovascular tissues for treatment. • Use of dendrimer/DNA complexes • Uniform size, high density, soluble, stable. • Direct injection or intracoronary delivery.

22. Enhanced expression of beta-galactosidase in electroporated nonvascularized grafts. • Graft treated as in group 12, Figure 1. • Graft treated as in group 4, Figure 1. • (Original magnification    40).