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Wu, Kuan-Yi. Properties of minD/ ParA superfamily. Structurally similar ATPase activity Polymerization Other playes dynamic oscillation. (ParA). YFP-MinD/ MinE-CFP. The relationship between players. Placement of division sites C: inhibitor of septation D: membrane recruitment of C

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Presentation Transcript
properties of mind para superfamily
Properties of minD/ ParA superfamily
  • Structurally similar
  • ATPase activity
  • Polymerization
  • Other playes
  • dynamic oscillation

(ParA)

the relationship between players
YFP-MinD/ MinE-CFPThe relationship between players

Placement of division sites

  • C: inhibitor of septation
  • D: membrane recruitment of C
  • E: topological specificity

DNA partition

  • A: recruited by B
  • B: recognize parS
  • S: cis-element in arrays
slide4
MinC+MinD

C MinC

DMinD

E MinE

Division inhibition

MinC concentration

Zone of division inhibition

MinE

Septal FtsZ ring

MinCDE polar zone

pole-to-pole

oscillation

Placement of Z-ring

slide5
1

ADP

MinD

MinD

- - -

+ + +

5

P

MinC

MinE

2

MinE

3

ATP

ATP

ATP

ATP

MinC

MinE

4

MinC

ATP

ATP

MinD

MinD

MinD

MinD

MinD

Molecular mechanism of the oscillating Min system

C-terminus

slide6
In vitro

In vivo

MinD

MinE

ATP

Phospholipid vesicle

ParA

ParB

ATP

slide8
MC1000/ pSOT1013

Live cell image

YFP

DIC

merge

DAPI

Oscillate with minD?  minD binding site

Move independently membrane targeting?

Existence of another proteins

In the presence or absence of parB

test on ori ter localization
Test on ori/ ter localization

YFP

DIC-YFP

DIC

CFP

IL2/ pLAU53

YFP

DIC-CFP

DIC

CFP

IL29/ pLAU53

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