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How Safe Is Seafood?

How Safe Is Seafood?. Harold Colon Environmental Testing Laboratories/ Freeport High School Mr. Edward Irwin Freeport High School Patty Els Environmental Testing Labs (ETL). Introduction.

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How Safe Is Seafood?

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  1. How Safe Is Seafood? Harold Colon Environmental Testing Laboratories/ Freeport High School Mr. Edward Irwin Freeport High School Patty Els Environmental Testing Labs (ETL)

  2. Introduction Mercury (Hg) a naturally occurring element is found as a liquid at room temperature. It is highly poisonous and can enter the body through the respiratory tract, the digestive tract or directly through the skin. It accumulates in the body, eventually causing severe illness or death. Not easily found alone in nature, it is primarily obtained from the mineral cinnabar (HgS). Mercury compounds and vapors can be used to produce useful materials such as thermometers, barometers and other scientific instruments. Mercury can also be used for making streetlights, fluorescent lamps and advertising signs…

  3. Introduction …Methylmercury [CH3Hg+] is the most toxic form. It is composed of a methyl group bonded to a mercury atom. Its exposure affects the immune system, alters genetic and enzyme systems, and damages the nervous system, including coordination and the senses of touch, taste, and sight. Exposure to methylmercury is usually by ingestion, and it is absorbed more readily and excreted more slowly than other forms of mercury. People are exposed to methylmercury almost entirely by eating contaminated fish and wildlife that are at the top of aquatic food chains. CH3Hg+ is introduced into the environment mainly through the burning of fossil fuels. Large fish such as marlins, sharks, swordfish and tuna tend to carry the highest concentrations of methylmercury and they are known to be of the largest dietary source.

  4. Sources of Mercury http://pubs.usgs.gov/fs/fs-016-03/images/fish.gif

  5. Materials & Methods • Materials • The PerkinElmer Flow Injection Mercury System (FIMS) • Reagent Grade Water • 5% Potassium Permanganate • Sodium Chloride Hydroxylamine Sulfate • Temperature Control Water Bath • Aqua Regia (ratio of 3:1 sulfuric acid and nitric acid) Mercury Extraction Method • Weigh 0.6g of sample • Add 5mL of RGW 5mL of aqua regia • Heat for 2 min @ 95°C, then cool • Add 50mL RGW 15mL 5% Potassium Permanganate (KMnO4) • Mix for 30 min @ 95°C • Cool, add 6mL of sodium chloride hydroxylamine sulfate • Filter • Analysis within 1-28 days • µg/g = (Mean FIMS reading [µg/L]) (0.081L) Gram of sample

  6. Problem • Is Atomic Absorption an accurate technique to measure Mercury levels in seafood? • Which type of seafood retains the most Mercury? • Given the type of seafood, Does Mercury accumulate differently in different parts of the animals? • Do plants have the ability to retain Mercury?

  7. Results • After analyzing several cans of tuna it was found that white albacore tuna carries approximately 5.73 times more Hg than that of light tuna. • The tuna analyzed contained 26 times more mercury than that of shrimp and crab • Preliminary studies were not able to show accumulation in plants, fruits and vegetables.

  8. Data

  9. Data

  10. Future Work • Analyze different parts of the fish in other species. • Analyze other species of fish suspected of retaining mercury. (Shark, Swordfish) • Find an experiment in which an explanation of why Hg is retained in certain parts of animals. • How Mercury binds to aquatic animals and compare it to those of humans.

  11. Conclusion • Preliminary results suggest that FIMS atomic absorption method is an accurate means of quantizing levels of Hg in seafood. • Tuna resulted in having a greater concentration than crab which in turn had more than shrimp. • White tuna carries a larger concentration of methylmercury than the light tuna • Neither of the plants tested showed the ability of retaining Mercury.

  12. References • IFT. 1973. Mercury in food. Scientific Status Summary,Expert Panel on Food Safety and Nutrition, Institute of Food Technologists, Chicago, IL. • Korns, R.F. 1972. The frustrations of Bettye Russow. Nutrition Today, 7(6):21-23. • The author is Robert J. Price, Ph.D., Seafood Technology Specialist, Department of Food Science & Technology, University of California, Davis, California 95616-8598 • [WHO] World Health Organization. 1990. Environmental health criteria 101. • Methylmercury. Geneva, Switzerland: WHO. Available from: http:// www.who.int/environmental_information/Air/Guidelines/ann4.htm. Accessed Sept 2003. • Gholam Reza Jahed Khaniki, Inteaz Alli, Ebrahim Nowroozi and Ramin Nabizadeh. Mercury Contamination in Fish and Public Health Aspects: A Review.Pakistan Journal of Nutrition 4 (5): 276-281, 2005 • Boischio AA, Hensel D. Fish consumption, fish lore, and mercury pollution--risk communication for the Madeira River people.Universidade Estadual de Feira de Santana, Bahia, Brazil. 2000 Oct;84(2):108-26.

  13. Acknowledgements • Dr. Sat Bhattacharya • Harlem Children Society • MSKCC • Mr. Ed Irwin • Patty Els • Juan Cuba • Ricardo Cuba • Carlos Strauss Thank You!!!

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