Chapter 8 DNA Fingerprinting and Forensic Analysis. Forensic science is the application of science to law previous technologies used photography, video cameras fingerprinting new technologies DNA fingerprints. What is a DNA fingerprint?.
PCR allows you to make millions of copies of the STR region from a single copy of DNA you recovered from crime scene.
Do any of the individuals compare with forensic sample?
G-C-A-T…….Wash away unreacted probeand add biotin-reactive enzyme
Colored product (spot lights up)
Lysis buffer causes vagina cells to lyse and
release DNA into solution
Centrifuge tube to sediment sperm cells, then decant supernatant to remove vagina DNA
Add lysis buffer 2
Lysis buffer causes remaining sperm cells
to lyse and release
DNA into solution
All provide accreditation to forensic laboratories
Proficiency testing of technicians
with PCR technology
then DNA fingerprinting
STRs of suspected Father
Is the suspect the father?