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Starch hydrolysis test is used to determine if the organism is capable of breaking down starch into maltose through the activity of the extra-cellular u03b1-amylase enzyme. Starch, the most important source of carbohydrate for humans, is a polysaccharide mixture of two polymers, amylose, and amylopectin, the latter being predominant.
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www.phdnest.com A QUICK INFORMATION GUIDE Starch Hydrolysis Test Objective, Principle, Procedure, & Results
www.phdnest.com Starch hydrolysis test is used to determine if the organism is capable of breaking down starch into maltose through the activity of the extra-cellular α-amylase enzyme. Starch, the most important source of carbohydrate for humans, is a polysaccharide mixture of two polymers, amylose, and amylopectin, the latter being predominant.
www.phdnest.com Starch Hydrolysis Test Objectives To determine the ability of the organism capable of hydrolyzing starch. To differentiate organisms based on their ability to hydrolyze starch with the enzyme, α- amylase. It aids in the differentiation of species from genera Corynebacterium, Clostridium, Bacillus, Bacteroides, Fusobacterium, and members of Enterococcus.
www.phdnest.com Starch Hydrolysis Test: Materials Heart infusion agar with 2% starch or Mueller Hinton agar (MHA) (MHA contains amylose thus can be used to test for starch hydrolysis, avoiding the purchase of additional media.) Gram’s iodine Sterile sticks or inoculating loops Incubator
www.phdnest.com Starch Hydrolysis Test: Test Procedure