P1823

1. P1823 Renat V. Adelshin1, Elena A. Sidorova1, Tatyana I. Borisova1, Ludmila S. Karan2, Sergey I. Belikov3, Nina B. Kulakova3, Evgeny I. Andaev1, Olga A. Nagibina4, Sergey V. Balakhonov1 1Irkutsk Antiplague Research Institute of Siberia and Far East of Rospotrebnadzor, Irkutsk, Russia; 2 Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia; 3 Limnological Institute of the Siberian Branch of the Russian Academy of Science, Irkutsk, Russia; 4 Chita Antiplague Station, Chita, Russia The area of tick-borne encephalitis (TBE) virus extends on the Euro-Asian continent from the Atlantic coast to Pacific Ocean. There are three genotypes of TBE virus: Far Eastern, Siberian and European. Tick-borne encephalitis foci have mosaic structure in Siberia where all three genotypes of TBE virus present with prevalence of the Siberian genotype. TBE morbidity in Chita region (Eastern Siberia) of Russia from 1998 to 2011 was 4.72 per 100 000 of population, lethality varied from 0 to 19.4 %. 23st European Congress of Clinical Microbiology and Infection Diseases, Berlin, Germany, 27 April – 30April 2013 Molecular genetic characterization of tick-borne encephalitis virus isolated from dead humans in Eastern Siberia Methods 98 Primorye (22 strains) 100 Two TBE virus strains isolated from died patients with meningoencephalitis at the Chita region were used in this investigation. The source of infection in both cases was taiga tick (Ixodes persulcatus). TBE virus strain Zabaikalye 1-98 was isolated from spinal marrow in 1998, strain Zabaikalye 11-99 was done from brain in 1999. RNA was isolated using the "Ribo-prep" kit, reverse transcription (RT) - with «Reverta-L-100» (ILS, Moscow). PCR was performed using «Sintol» kit (Moscow) and original primers. PCR products were sequenced with ABI Prism Big Dye Terminator Kit v.1.1 and ABI Prism DNA Sequencer 3500xL. Nucleotide sequences were analyzed by the BioEdit v.7.0.9.0 program. The phylogenetic analysis was performed by MEGA v.5. Oshima 5-10 100 Shkotovo-94 Irkutsk-1861 100 Primirye-89 91 Primorye-633 100 100 SOFJIN-HO FarEasterngenotype 80 Primorye (7 strains) 99 Primorye-196 100 100 Primorye-52 Glubinnoe/2004 100 Zabaikalye 1-98 98 Svetlogorie 100 Kavalerovo 100 Results Senzhang 70 178-79 High identity (94.2 %) of full genome sequence (10554 bp) of TBE virus strain Zabaikalye 1-98 and the prototype strain of Far Eastern genotype (Sofjin-HO) was identified. Phylogenetic analysis showed that Zabaikalye-1-98 strain followed in a group of strains isolated from brain of died patients in Far East and China. Sequence analysis of TBE virus strain Zabaikalye 11-99 (10063 bp) revealed its belonging to the Siberian genotype and identity to prototype strain Vasilchenko (97.6 %). On a phylogenetic tree the nearest (identity – 99.9 %) strain to Zabaikalye 11-99 was a Cht-653 isolate from a brain of died patient at the neighbouring area of Chita region in 1995. 886-84 100 EK-328 86 100 Latvia-1-96 Est54 Cht-22 100 100 Irkutsk-12 Siberiangenotype Kolarovo-2008 100 Zabaikalye 11-99 100 Cht-653 100 VASILCHENKO 100 Aina Toro-2003 Conclusion Est3476 77 99 Kumlinge A52 100 Thus, there are two genotypes (Far Eastern and Siberian) of TBE virus on the same territory of Chita region and both of them cause the identical disease (meningoencephalitis) with a lethal outcome. Salem Europeangenotype NEUDOERFL 93 Hypr AS33 99 Joutseno Langat virus Address: Irkutsk Antiplague Research Institute of Siberia and Far East, Trilisser St., 78, Irkutsk, Russia 664047 Email: adelshin@gmail.com ML-tree, Jukes-Cantor model, polyprotein gene 0.05