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Log10 (pvalue)

1.5 Fold. B. C. Ctr. Exp. 1036. 7. 6. 5. 1036. 4. 3. 2. 1. -4. -2. 0. 2. 4. Log2 1.5 Fold change. D. 180. 138. 139. 73. 156. Replicate 1 2 3 4 1 2 3 4. (low) relative expression levels (high). A. Pro-inflammatory cytokines.

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Log10 (pvalue)

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  1. 1.5 Fold B C Ctr. Exp. 1036 7 6 5 1036 4 3 2 1 -4 -2 0 2 4 Log2 1.5 Fold change D 180 138 139 73 156 Replicate 1 2 3 4 1 2 3 4 (low) relative expression levels (high) A Pro-inflammatory cytokines RNA Microarray qRT-PCR TNF-α and IL-1β Intervertebral disc NP Osmolarity Nutrition Protein isolation Western blot Log10 (pvalue)

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