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Gene Regulation Study: Leptin and ERα Interaction in MDA-MB-231 Cells

This schematic outlines an experimental setup to identify genes regulated by leptin and enhanced by ERα through microarray analysis in MDA-MB-231 cells. Transfected with Ob-RL and HEG0 cDNAs, cells were stimulated with leptin for 9 hours to study gene expression changes.

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Gene Regulation Study: Leptin and ERα Interaction in MDA-MB-231 Cells

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  1. Figure S1 sample 1: E+/L- sample 2: E+/L+ sample 3: E-/L- sample 4: E-/L+ Fig. S1. Schematic overview of the experimental set up to identify leptin-regulated and ERα-enhanced genes by microarray analysis. All MDA-MB-231 cells were transfected with Ob-RL cDNA and either an HEG0 cDNA construct tooverexpress ERα (sample 1 and 2) or with the corresponding empty vector (sample 3 and 4) followed by leptin stimulation for9 h (sample 2 and 4). E, ERα; L, leptin; +, positive; -, negative.

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