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MALDI TOF-TOF. Digest Complex Protein Mixture. Protein Spot In-gel Digestion. MDLC. 2 DGE. MS/MS Spectra. Allow for PTM. In-House MASCOT Search ( Macaca Mulatta ). Protein ID. No PTM. Deconvolute. No PTM. De novo Sequence. PEAKS De novo. No Protein ID. Allow for PTM.

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2 dge

MALDI

TOF-TOF

Digest

Complex Protein

Mixture

Protein Spot

In-gel

Digestion

MDLC

2 DGE

MS/MS Spectra

Allow for

PTM

In-House

MASCOT Search

(Macaca Mulatta)

Protein ID

No PTM

Deconvolute

No PTM

De novo

Sequence

PEAKS

De novo

No

Protein ID

Allow for

PTM

Search for

homologous protein

Change In-House

Database For Future

MASCOT Search

Compilation of

Protein Database for

Macaca Mulatta

PEAKS

Search Engine

Protein ID

Probable

Errors in

de novo

SPIDER

Search Engine

Protein ID


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