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Regulation of Ca++ Homeostasis in Female Rat Hearts: Hormonal Influence and Functional Correlation

Investigating the relationship between heart function and hormones in female rats, examining the expression of Ca++ homeostasis proteins under different stress conditions. The study explores the impact of acute and oxidative stress on the heart and aims to ascertain the correlation between hormone-dependent expression of Ca++ regulating proteins and heart function. The research involves comparing intact and ovariectomized rats under sedentary or exercise conditions, subjected to various stressors like doxorubicin and dexrazoxane. Heart extraction and homogenization techniques are used, followed by Western blot analysis using a densitometer.

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Regulation of Ca++ Homeostasis in Female Rat Hearts: Hormonal Influence and Functional Correlation

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  1. Regulation of Ca++ Homeostasis in the Hearts of Female Rats Sarah-Neïel Barama October 19th 2006 Supervisor: Dr. Chalifour (LDI)

  2. Background & Hypothesis • Relationship b/w ♥ & hormones • Ca++ Homeostasis proteins (NCX1, SERCA2a, PLB, CSQ, CRT…) • Contract = Ca++ → cytosol • Relax = Ca++ → SR & ECM • Acute Stress: exercise • Oxidative stress : Doxorubicin ± Dexrazoxane Hyp: Expression of Ca++ regulating proteins is . Hormone-dep and is correlated to ♥ fct

  3. Method • Intact VS Ovariectomy • Sedentary or Exercised • PBS or DOX or DOX:DEX • ♥ Extraction & Homogenisation in RIPA • Western Blot, densitometer

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