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09/07/ 17 (yy/mm/dd)

09/07/ 17 (yy/mm/dd). Experimenter Kashima,Shihoya. Note-taker Kashima. Title. 7/17 Miniprep Kit, Insert check. Flow Chart. Miniprep Kit Insert check. 1-1(2-A-4+2-O-2) ①②③. iGEM DNA parts kits. Miniprep Kit. 1.5ml tube each x1. 6F freezer. Miniprep Kit.

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09/07/ 17 (yy/mm/dd)

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  1. 09/07/ 17 (yy/mm/dd) Experimenter Kashima,Shihoya Note-takerKashima Title 7/17 Miniprep Kit, Insert check Flow Chart • Miniprep Kit • Insert check 1-1(2-A-4+2-O-2)①②③ iGEM DNA parts kits Miniprep Kit 1.5ml tube each x1 6F freezer

  2. Miniprep Kit Cultured sample (090716 14:00 ～09071709:00 ) ;1-1①②③ Centrifuge 4℃,15000 ×g, 2min twice Remove the top of layer Photo (Fig.1) P1 250ul P2 250ul N3 350ul Centrifuge 4℃,15000 ×g, 10min Apply the top of layer to column Centrifuge RT, 10sec and remove through Buffer PB 500ul Centrifuge RT, 10sec and remove through Buffer PE 650ul Centrifuge RT, 10sec and remove through Centrifuge 4℃,15000 ×g, 1min Exchange tube (remove C2H5 OH) Milli Q 50ul RT 1min Centrifuge 4℃,15000 ×g, 1min Collect through and measure concentration (Discussion)

  3. Result of Miniprep Kit (Discussion)

  4. Description (Insert check) Restriction Enzyme Digestion 37 ℃　incubate ( time: 090717 10:20～090717 10:55) Electrophoresis Gel :Agarose Time : 11:05～ 11:40 Voltage: 100V Maker: 1Kbp (Discussion)

  5. Result of Insert check (Discussion) We checked that①,②and③colonies have the plasmid incerted 1-1(2-A-4+2-O-2 :4.7kbp)

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