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Antimicrobial activity of Nigella sativa oil against multi-drug resistant Pseudomonas aerugenosa from clinical speci

Aligarh Muslim University. Antimicrobial activity of Nigella sativa oil against multi-drug resistant Pseudomonas aerugenosa from clinical specimens. Mohd. Tariq Salman, Rahat Ali Khan and Indu Shukla* Departments of Pharmacology and Microbiology* Jawaharlal Nehru Medical College.

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Antimicrobial activity of Nigella sativa oil against multi-drug resistant Pseudomonas aerugenosa from clinical speci

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  1. Aligarh Muslim University Antimicrobial activity of Nigella sativa oil against multi-drug resistant Pseudomonas aerugenosa from clinical specimens. Mohd. Tariq Salman, Rahat Ali Khanand Indu Shukla* Departments of Pharmacology and Microbiology* Jawaharlal Nehru Medical College

  2. Introduction • Traditional healers have long used plants toprevent/cure infective conditions. • Nigella sativa L. (Kalonji) has been used for centuries for treatment of various ailments, including infections. • Its seeds, commonly used in recipes in Asian countries are reported to possess many medicinal properties. • Its crude extracts, oil possess antibacterial activity against many pathogenic bacteria. • Objective: To study effect of N. sativa essential oil against standard and multidrug resistant strains of Pseudomonas aerugenosa.

  3. Materials and Methods • N. Sativa oil(Mohammedia products, Hyderabad) tested in various dilutions by disc agar diffusion technique using impregnated filter paper discs on inoculated Muellar Hinton agar plates against P.Aerugenosa (ATCC 27853 and NCTC 10662) and 22 clinical isolates of pseudomonas obtained from pus, blood, ear discharge and conjunctival swab of patients attending J.N.M.C. Hospital, Aligarh during 2003 & 2004. • Out of the clinical isolates taken, 3 were resistant to 2-4 antibiotics, 6 to 5-7 antibiotics, 8 to 8-10 antibiotics and 5 to 11-13 antibiotics. • Resistance was highest for Ampicillin, Gentamicin, Ciprofloxacin, Amikacin, Ceftazidime, Cefotaxime, Ofloxacin and Ceftriaxone.

  4. Observations Effect of N. sativa oil against standard strains.(Mean diameters of zones of inhibition in mm around 6 mm discs impregnated with Oil or commercial diagnostic antibiotic sensitivity testing disc)

  5. Effect of N. sativa oil against clinical isolatesMean diameters of zones of inhibition in mm around 6 mm discs impregnated with Oil

  6. A- Ampicillin, Ak- Amikacin, Co- Cotrimoxazole, Clr- Cefaclor, Cp- Ciprofloxacin, Cpz- Cefoperazone, Ctr- Ceftriaxone, Ctx- Cefotaxime, Ctz- Ceftazidime, G- Gentamicin, Gt- Gatifloxacin, I- Imipenem, O- Ofloxacin, P- Piperacillin, R- Roxithromicin, S- Sparfloxacin, T- Tetracycline, To- Tobramycin,

  7. ResultsActivity of N. sativa oil was seen: • upto a dilution of 1:50 and 1:100 respectively against P.aerugenosa ATCC 27853 and NCTC 10662 • upto 1:50 dilution against 1 strain resistant to 6 antibiotics. • upto 1:10 dilution against 5 strains resistant to 2-11 antibiotics. • in undiluted state against 6 strains resistant to 6-11 antibiotics. • Conclusion:N. sativa oil is active against standard as well as multi-drug resistant strains of P.aerugenosa and may be used therapeutically in susceptible cases.

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