1 / 10

Enzymatic Attacks on Nitrogen-Containing Substrates: Urease, Gelatinase, and Nitrate Reductase

This article delves into the enzymatic mechanisms of urease, gelatinase, and nitrate reductase that target nitrogen-containing substrates. Urease hydrolyzes urea into ammonia and carbon dioxide, with pH indicators revealing acidic (yellow) or basic (pink) conditions in urea broth. Gelatinase transforms solid gelatin into a liquid state, and its test is performed on Tryptic Soy Gelatin Agar. Lastly, nitrate reductase facilitates the conversion of nitrate to nitrite and water, particularly in facultative bacteria using nitrate broth.

kevyn
Download Presentation

Enzymatic Attacks on Nitrogen-Containing Substrates: Urease, Gelatinase, and Nitrate Reductase

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Ex. 22 Enzymatic Attacks on Nitrogen Containing Substrates

  2. Enzymes that attack “N” substrates • 1. Urease • 2. Gelatinase • 3. Nitrate Reductase

  3. Urease Urea + water ammonia + carbon dioxide Urea Broth Urea pH Indicator Acidic = Yellow Basic = Pink

  4. UreaseTest

  5. Gelatinase Gelatin (solid) Gelatin (liquid) Tryptic Soy Gelatin Agar

  6. Gelatinase Test

  7. Nitrate Reductase Nitrate Nitrite + water Facultative Bacteria Nitrate Broth

More Related