ex 22 enzymatic attacks on nitrogen containing substrates n.
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Ex. 22 Enzymatic Attacks on Nitrogen Containing Substrates. Enzymes that attack “N” substrates. 1. Urease 2. Gelatinase 3. Nitrate Reductase. Urease Urea + water ammonia + carbon dioxide Urea Broth Urea pH Indicator Acidic = Yellow Basic = Pink.

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enzymes that attack n substrates
Enzymes that attack “N” substrates
  • 1. Urease
  • 2. Gelatinase
  • 3. Nitrate Reductase
slide3

Urease

Urea + water ammonia + carbon dioxide

Urea Broth

Urea

pH Indicator

Acidic = Yellow

Basic = Pink

slide6

Gelatinase

Gelatin (solid) Gelatin (liquid)

Tryptic Soy Gelatin Agar

slide8

Nitrate Reductase

Nitrate Nitrite + water

Facultative Bacteria

Nitrate Broth