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Percentage of Venus positive HEK293_M2 cells after lentivirus infection at MOI 0.3 (pLD-T-hORFeome-IRES-Virus-STOP, living cells only). 250. 250. 250. 250. 250. 250. 200. 200. 200. 200. 200. 200. 150. 150. 150. 150. 150. 150. 100. 100. 100. 100. 100. 100. a. 1.7%. 1%.

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Percentage of Venus positive HEK293_M2 cells after lentivirus infection at MOI 0.3 (pLD-T-hORFeome-IRES-Virus-STOP, living cells only)

250

250

250

250

250

250

200

200

200

200

200

200

150

150

150

150

150

150

100

100

100

100

100

100

a

1.7%

1%

1.9%

FSC-H

FSC-H

FSC-H

50

50

50

50

50

50

No virus

0

0

0

0

0

0

0

0

0

0

0

0

101

101

101

101

101

101

102

102

102

102

102

102

103

103

103

103

103

103

104

104

104

104

104

104

SSC-H

SSC-H

SSC-H

12 hours dox

24 hours dox

48 hours dox

b

32%

42%

44%

FSC-H

FSC-H

FSC-H

Virus

SSC-H

SSC-H

SSC-H

Additional File 5