1 / 16

Cell-Scaffold interaction

Manifestation of Novel Social Challenges of the European Union in the Teaching Material of Medical Biotechnology Master’s P rogrammes at the University of Pécs and at the University of Debrecen Identification number : TÁMOP-4.1.2-08/1/A-2009-0011.

keena
Download Presentation

Cell-Scaffold interaction

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat theUniversity of Pécs and at the University of Debrecen Identificationnumber: TÁMOP-4.1.2-08/1/A-2009-0011

  2. Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat theUniversity of Pécs and at the University of Debrecen Identification number: TÁMOP-4.1.2-08/1/A-2009-0011 Dr. Judit Pongrácz Threedimensionaltissuecultures and tissueengineering – Lecture 11 Cell-Scaffoldinteraction

  3. ScaffoldsI • Pre-made network of biomaterial • One cell type seeded on one scaffold • Several cell types seeded on a scaffold simultaneously or sequentially

  4. ScaffoldsII Fleece Fusedbeeds CO2 Fusedirregular Fusedcubeporogen Cubeporogen

  5. Cell line cells nestling on a scaffold A549 (lung adenocarcinoma cell line)

  6. Matrigel® • Matrigel: • is the trade name for a gelatinous protein mixture secreted by mouse sarcoma cells • This mixture resembles the complex extracellular environment found in many tissues • Matrigel is marketed by BD Biosciences

  7. Primary SAEC(Small Airway Epithelial Cells) in matrigel

  8. Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat theUniversity of Pécs and at the University of Debrecen Identification number: TÁMOP-4.1.2-08/1/A-2009-0011 Dr. Judit Pongrácz Threedimensionaltissuecultures and tissueengineering – Lecture 12 Biofactors

  9. Biofactors Adjacentcell Wnt Wnt Cytosol Frizzled FGF GSK3 BMP Disheveled FGF receptor b-TrCP b-catenin accumulation b-catenin degradation PI3K TGF-b PLCg Nucleus Jaggedor Delta-like ligands BMP and TGF-breceptor TCF SMAD ATF2 Elk1 TAK Hey ATF2 c-fos c-jun Notch Proliferation Differentiation Migration ICD HES

  10. Main growthfactors (GFs) used in tissue engineering • VEGF– vascularendothelialgrowthfactor • KGF – keratinocytegrowthfactor • TGF-b– transfrominggrowthfactorbeta • BMP– bonemorphogenic protein • FGF– fibroblastgrowthfactor • PDGF– platelet-derivedgrowthfactor

  11. VEGF • Primarilyinendothelialcells • Dimericglycoprotein • Foursplicevariants • Receptors: VEGFR-1 and VEGFR-2

  12. TGF-b • Superfamilymember • MW 21 kDa • Synthesizedbyplatelets and macrophages • Inducesdifferentiationinmultiplecelltypes of varioustissues

  13. BMP • Osteoinductivepeptidefamily • BelongstotheTGF-bsuperfamily • MW 25-30 kDa

  14. FGF • 23 members of thefamily • Multiplefunctionsonmultiplecelltypes • Human FGF-2 18 kDanon-glycosylatedpolypeptidebindstoheparin and heparansulfate

  15. PDGF • Existsas a dimer • Twochains MW 14-17 kDaeach • Synthesizedbyplatelets, macrophages, endothelium, connectivetissue • Effects: Mitogenesis, angiogenesis, macrophageactivation

  16. Delivery of growthfactors • Injected • Diffusedfromscaffolds • Releasedfromgels • Geneticallyengineeredintocells

More Related