CD molecules

1. CD molecules & Disease

2. 制作团队： 资料：冯华 樊慧慧 崔晓霈 李金枝 何平 鲁建华 张泽立 整理：冯华 翻译：崔晓霈 李金枝 樊慧慧 制作：鲁建华 何平 2000级七年制一班

3. CD molecules & disease • Concept CD molecules,which are also called leukocyte differentiation antigen,are the surface markers appear or disappear on leukocytes (also include platelet,blood vessel endothelial cell etc) during the process of differentiation and activation.

4. CD molecules & disease According to monoclonal antibody identification method,the same differentiated antigen that can be recognized by monoclonal antibody coming from different labs are called CD (cluster of differentiation)

5. CD molecules & disease • Structure Most CD molecules are transmembrane protein or glycoprotein,including extramembrancous,transmembranous and intracellular areas.

6. CD molecules & disease • Classifications CD molecules are numbered according to the time of discovery.So far,they have been named from CD1 to CD166.

8. CD molecules & disease All of them are classified approximately into 9 groups: T cell, B cell, activating cell, myeloid cell, NK/non-lineage cell, platelet, endoepithelial cell, adhesion molecule and cytokine receptor .

9. CD molecules & disease • Function 1. CD molecules take part in the recognition between immune cells and Ag or other immune cells.They also participate the activation,proliferation differentiation and immune function.

11. CD molecules & disease 2.Regulate the differentiation and migration of hemopoietic cells. 3.Participate inflammation. 4.Cell migration. eg.cancer matastasis

13. CD molecules & disease • Cdmolecules and disease 1.CD molecules’normal functions: 1)CD molecules related to activation of T cell ,TCR/CD3 Complex,CD4 Co- receptor,CD8,CD2,CD58,CD28- Co- stimulators

16. CD molecules & disease 2).CD molecules related to inflammatory reactions. Firstly:CDb2I and CDb2E mediate neutrophiles to roll and combine,the monoclonal Ab that until-selection can inhibit the reaction dramatically When passing through endothelium, CD4/CD18 interacts with its ligand,playing an important role.

17. CD molecules & disease 3).CD associated with blood cloting : eg: GP16

18. CD molecules & disease 2.Disease: 1)EB: LFA-3 lacking 2)Paroxys nocturnal hemoglosinvia（周期 夜现性血尿 ） CD6+ CD28+ T cell MHC CD8+ CD28- inhibit antibody

20. CD molecules & disease 3)Disease associated with intracellular signals transmission of CD,CD3,CD45,CD115,CD125,CD117,CD130,their signals’abnormal transduction cause hyperplastic disease,what’s more,they are intimately involved in tumor,papillomas,psoriasis,etc.

21. CD molecules & disease 4)CD and tumors (1) CD44 and cancer (tumor) metastasis eg: * melanomatous cells express CD44 *The inflammayion products and some cytokines promotes local vessels endothelial cells to express more CAMs,which are helpful to the adhesion of tumor cells

22. CD molecules & disease (2)Stimulation of CD40 can inhibit the reproduction of tumor cells directly,promote apoptosis and Fasgene expression (3)The interaction between LFA-I and ICAM-1 mediate the contant between NK cells and tumor cells.

23. CD molecules & disease ICAM－2 Mutations Affecting LFA-1 Binding

24. CD molecules & disease 5)CD and HIV. HIV enters the T cell by combining with CD4 molecules on T cells.In the blood of the person infected by HIV-I,CD4 cell appears positive.The CD4+ cells’obvious decrease is associated with the disease deterioration.

25. CD molecules & disease • HIV infecting normal human’s tissues

27. CD molecules & disease 6)CD and GSS (Granulomatous slack skin肉芽肿性皮肤松弛症） Mainly CD4+ CD8- T cells and CD45RO+ T cells antigen loss in different measures. CD4+/CD45RO+ T cells, CD3, CD5 and CD7 (marker of T cells) loss.

28. CD molecules & disease 7)CD and CO poisoning *CO poisoning damage the endothelial cells *The activation of platelets increase and CD116, CD18 are highly expressed. *Thrombus in cerebral microarterial vessels is induced to form.

29. CD molecules & disease 8)CD and diabetes -I Immune tolerance deficiency of insular specific T cells is due to blockage of ICAM-I/CFA-1 and loss CD8+ T cells at the beginning of immune response,which lead to insular antigen tolerance deficiency.

30. CD molecules & disease 9)Others *Increasing of activators of platelet-CD62P,CD61,and CD41,especially CD62P,are closely related to the disease such as coronary heart disease,cerebral infarction,hypertension,etc.

31. CD molecules & disease *Associated with endometriosis.（子宫内膜异位） eg:Periodical change of CD45+ cell group become abnormal. *The disease such as asthma,rhinitis,etc are possibly associated with actiration of CD8+ and CD127+ cells

32. CD molecules & disease Anusual expression of CAM and SLE *SLE (systemic lupus erythematosus系统性红斑狼疮) *CD11a and CD18 expression decline agree with CD4+ T cell while increase with CD8+ T cell. *Expression of CD54 on CD20+ cells increase

33. CD molecules & disease • CD molecules and clinical treatment 1.CD34 and Aplastic anemia (再生障碍性贫血） CD34 is expressed in most acute lympholeukemia (急性淋巴细胞性白血病） Detection of CD34 is helpful to the study of Aplastic anemia

34. CD molecules & disease 2.CD34 as marks of T cells MACS is used to select and separate CD34+ T cells from peripheral blood Reduced T cell-load is good for autoimmune disease

35. CD molecules & disease 3.Application of anti-CD40 McAb anti-CD40 McAb can significantly inhibit the development of Osseous rheumatism（类风湿性关节炎）

36. CD molecules & disease 4.Application of anti-CD3- Ab anti-CD3-Ab is now widely used to cure enograft rejection and is efficient bispecific anti-CD3-/HSV Ab for treatment of the immunodeficient newborn infected by HSV

37. CD molecules & disease 5.Anti-CD3 McAb 1)anti-CD3McAb in mice can reverse enograft rejection 2)Mechanism *anti-CD3 McAb can bind with CD3 molecules on T cells

38. CD molecules & disease *The interaction interfere with the transduction of activating signals which results in cytokine releasing *The proliferation and functions of T cells be blocked

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