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Introduction to the Microscope. Nosepiece. Types Care Parts Using. Types of Microscopes. First microscopes were simple scopes One magnifying lens (i.e. one magnifying glass) Compound Microscopes Two magnifying lenses & a source of light Dissection Microscopes

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Types care parts using

Introduction

to the Microscope

Nosepiece

Types

Care

Parts

Using


Types care parts using

Types of Microscopes

  • First microscopes were simple scopes

    • One magnifying lens (i.e. one magnifying glass)

  • Compound Microscopes

    • Two magnifying lenses & a source of light

  • Dissection Microscopes

    • Low magnification (cannot see cells)

    • Used for dissection to get a better look at the larger specimen

  • Scanning electron microscope (SEM)

    • Extremely detailed 3D views of the surface of the specimen (think of “S” for surface)

    • Specimens are coated with metal

  • Transmission electron microscope (TEM)

    • Electrons pass through the specimen - can view internal structure (think of “T” for through)

    • Specimen must be sliced into an extremely thin section - 20-100 nm and stained with metals


  • Sem images
    SEM images

    Bacteria

    Termite

    Blood cell

    Ballpoint pen


    Tem images
    TEM images

    Mitochondrion

    Bacterium

    Rough ER

    Chloroplast


    Types care parts using

    Microscope Care

    • Always carry with 2 hands

    • Never allow cords to hang over the counter

    • Do not force knobs

    • Always use the fine focus if on any power higher than scanning (4X)

    • Loosely wrap the cord around the scope for storage

    • Never place on top of the vent in front of your station!


    Types care parts using

    Microscope Parts

    Ocular Lens- Eyepiece

    RevolvingNosepiece

    Arm

    ObjectiveLens

    Stage

    Stage Clips

    Coarse Adjustment Focus

    Diaphragm

    Fine Adjustment Focus

    Light Source

    Base


    Types care parts using

    Parts of the

    Microscope Slide

    Coverslip

    Slide


    Types care parts using

    Using the Microscope

    • Turn the microscope on

    • Lower stage

    • Click Nosepiece to the shortest setting (4X)

    • Place the Slide on the Microscope

    • Use Stage Clips

    • Look into the Eyepiece

    • Use the Coarse Focus to slowly bring specimen into focus

    • Bring specimen into focus using Fine

      Focus knob

    • Adjust diaphragm if necessary


    Types care parts using

    Using High Power

    • Follow steps to focus using low power

    • Carefully turn the nosepiece to the next longest objective

    • Do NOTuse the Coarse Focusing Knob

    • Use the Fine Focus Knob to bring the specimen into focus


    When finished observing
    When Finished Observing

    • Bring stage all the way down

    • Turn nosepiece so that smallest objective is directly over the slide

    • Carefully remove the slide!


    Microscope terms
    Microscope Terms

    • Magnification

    • Resolution

    • Field of View

    • Depth of Focus

    • Image Inversion


    Magnification
    Magnification

    • Making an image appear larger than its actual size

    • For example: magnification value of 100X means an image appears 100 times larger than its actual size

    • Total Magnification = objective mag. x ocular mag.

      • Example: 40X mag. (objective) times 10X mag. (ocular) = total of 400X mag.


    Resolution
    Resolution

    • The ability of a microscope to show the details of an object, ability to distinguish between 2 lines

    • The higher the resolution the clearer the image

      • Both high magnification and good resolution are needed to view the details of extremely small objects clearly.


    Focusing on an object
    Focusing on an Object

    • Remember: Locate and Focus on your object with the scanning (smallest) objective lens first

    • This provides:

      • Greater Depth of Focus – depth of 3D objects (layers)

      • Greater Field of View – how much of the image you

        can see