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Introduction to the Microscope. Nosepiece. Types Care Parts Using. Types of Microscopes. First microscopes were simple scopes One magnifying lens (i.e. one magnifying glass) Compound Microscopes Two magnifying lenses & a source of light Dissection Microscopes

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Types care parts using


to the Microscope






Types care parts using

Types of Microscopes

  • First microscopes were simple scopes

    • One magnifying lens (i.e. one magnifying glass)

  • Compound Microscopes

    • Two magnifying lenses & a source of light

  • Dissection Microscopes

    • Low magnification (cannot see cells)

    • Used for dissection to get a better look at the larger specimen

  • Scanning electron microscope (SEM)

    • Extremely detailed 3D views of the surface of the specimen (think of “S” for surface)

    • Specimens are coated with metal

  • Transmission electron microscope (TEM)

    • Electrons pass through the specimen - can view internal structure (think of “T” for through)

    • Specimen must be sliced into an extremely thin section - 20-100 nm and stained with metals

  • Sem images
    SEM images



    Blood cell

    Ballpoint pen

    Tem images
    TEM images



    Rough ER


    Types care parts using

    Microscope Care

    • Always carry with 2 hands

    • Never allow cords to hang over the counter

    • Do not force knobs

    • Always use the fine focus if on any power higher than scanning (4X)

    • Loosely wrap the cord around the scope for storage

    • Never place on top of the vent in front of your station!

    Types care parts using

    Microscope Parts

    Ocular Lens- Eyepiece





    Stage Clips

    Coarse Adjustment Focus


    Fine Adjustment Focus

    Light Source


    Types care parts using

    Parts of the

    Microscope Slide



    Types care parts using

    Using the Microscope

    • Turn the microscope on

    • Lower stage

    • Click Nosepiece to the shortest setting (4X)

    • Place the Slide on the Microscope

    • Use Stage Clips

    • Look into the Eyepiece

    • Use the Coarse Focus to slowly bring specimen into focus

    • Bring specimen into focus using Fine

      Focus knob

    • Adjust diaphragm if necessary

    Types care parts using

    Using High Power

    • Follow steps to focus using low power

    • Carefully turn the nosepiece to the next longest objective

    • Do NOTuse the Coarse Focusing Knob

    • Use the Fine Focus Knob to bring the specimen into focus

    When finished observing
    When Finished Observing

    • Bring stage all the way down

    • Turn nosepiece so that smallest objective is directly over the slide

    • Carefully remove the slide!

    Microscope terms
    Microscope Terms

    • Magnification

    • Resolution

    • Field of View

    • Depth of Focus

    • Image Inversion


    • Making an image appear larger than its actual size

    • For example: magnification value of 100X means an image appears 100 times larger than its actual size

    • Total Magnification = objective mag. x ocular mag.

      • Example: 40X mag. (objective) times 10X mag. (ocular) = total of 400X mag.


    • The ability of a microscope to show the details of an object, ability to distinguish between 2 lines

    • The higher the resolution the clearer the image

      • Both high magnification and good resolution are needed to view the details of extremely small objects clearly.

    Focusing on an object
    Focusing on an Object

    • Remember: Locate and Focus on your object with the scanning (smallest) objective lens first

    • This provides:

      • Greater Depth of Focus – depth of 3D objects (layers)

      • Greater Field of View – how much of the image you

        can see