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Lab 7, PCA of small data set Beeromics , groups 3 and 4. Nils Nyberg NPR, Department of Drug Design and Pharmacology. Differences and similarities among beers . 500 µL Beer + 500 µL 1 M phosphate buffer ( pD 7.4), D 2 O

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Lab 7, PCA of small data set Beeromics , groups 3 and 4


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lab 7 pca of small data set beeromics groups 3 and 4
NTDR, 2012

Lab 7, PCA of small data set Beeromics, groups 3 and 4

Nils Nyberg

NPR, Department of Drug Design and Pharmacology

differences and similarities among beers
Differences and similarities among beers
  • 500 µL Beer+ 500 µL 1 M phosphate buffer (pD 7.4), D2O
  • 6 different beers, 4 tubes/sample, analyzed twice = 48 spectra
  • Water signal suppression by presaturation
pca on whole range
PCA on whole range
  • Calibrated and Normalized using the internal reference signal from DSS at 0.0 ppm
  • 218 buckets
  • 0.04 ppm/bucket
  • Pareto scaled
pca on whole range1
PCA on whole range
  • Loadings: Differences in sugar signals
pca on aromatic range 5 8 9 0 ppm
PCA on aromatic range; 5.8-9.0 ppm
  • Calibrated and Normalized using the internal reference signal from DSS at 0.0 ppm
  • 319 buckets
  • 0.01 ppm/bucket
  • Pareto scaled
pca on aromatic range 5 8 9 0 ppm1
PCA on aromatic range; 5.8-9.0 ppm
  • Calibrated and Normalized using the internal reference signal from DSS at 0.0 ppm
  • 319 buckets
  • 0.01 ppm/bucket
  • Pareto scaled
pca on aromatic range 5 8 9 0 ppm2
PCA on aromatic range; 5.8-9.0 ppm
  • Calibrated and Normalized using the internal reference signal from DSS at 0.0 ppm
  • 319 buckets
  • 0.01 ppm/bucket
  • Pareto scaled
pca on aromatic range
PCA on aromatic range
  • Loadings: Specific signals highlighted, but not possible to identify signals