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Utilize molecular markers for precise cDNA analysis with ActRI internal standard. Native ActRI cDNA spot density (x105) aids in accurate measurements.
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A. Molecular Negative 0.01 0.1 1 10 pg cDNA Marker control 0.5 0.5 0.5 0.5 pg IS 1018bp Log (ActRI/Internal Standard) -1 0 1 652bp 492bp 506bp 0.01 0.1 1 10 Native ActRI cDNA (pg) B. 16 14 12 0.5 1 2 4 ml cDNA Spot density(X105) 10 524bp 8 6 4 2 0.5 1 2 4 cDNA (ml)