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Messenger RNA. Chapter 7. 7.1 Introduction. Figure 7.1. 7.2 mRNA Is Produced by Transcription and Is Translated. Only one of the two strands of DNA is transcribed into RNA. Figure 7.2. 7.3 Transfer RNA Forms a Cloverleaf. A tRNA has a sequence of 74 to 95 bases.

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messenger rna

Messenger RNA

Chapter 7

7 2 mrna is produced by transcription and is translated
7.2 mRNA Is Produced by Transcription and Is Translated
  • Only one of the two strands of DNA is transcribed into RNA.

Figure 7.2

7 3 transfer rna forms a cloverleaf
7.3 Transfer RNA Forms a Cloverleaf
  • A tRNA has a sequence of 74 to 95 bases.
    • It folds into a cloverleaf secondary structure with four constant arms.
      • Longer tRNAs have an additional arm.

Figure 7.4

slide5
tRNA is charged to form aminoacyl-tRNA by forming an ester link between the:
    • 2′ or 3′ OH group of the adenylic acid at the end of the acceptor arm
    • COOH group of the amino acid

Figure 7.3

slide6
The sequence of the anticodon is solely responsible for the specificity of the aminoacyl-tRNA.

Figure 7.5

7 4 the acceptor stem and anticodon are at ends of the tertiary structure
7.4 The Acceptor Stem and Anticodon Are at Ends of the Tertiary Structure
  • The cloverleaf forms an L-shaped tertiary structure with:
    • the acceptor arm at one end
    • the anticodon arm at the other end

Figure 7.6

Figure 7.7

7 5 messenger rna is translated by ribosomes
7.5 Messenger RNA Is Translated by Ribosomes
  • Ribosomes are characterized by their rate of sedimentation
    • 70S for bacterial ribosomes and 80S for eukaryotic ribosomes.
  • A ribosome consists of:
    • a large subunit (50S or 60S for bacteria and eukaryotes)
    • a small subunit (30S or 40S)

Figure 7.8

slide9
The ribosome provides the environment in which aminoacyl-tRNAs add amino acids to the growing polypeptide chain.
  • A ribosome moves along an mRNA from 5′ to 3′.
7 6 many ribosomes bind to one mrna
7.6 Many Ribosomes Bind to One mRNA
  • An mRNA is simultaneously translated by several ribosomes.
  • Each ribosome is at a different stage of progression along the mRNA.

Figure 7.9

7 7 the life cycle of bacterial messenger rna
7.7 The Life Cycle of Bacterial Messenger RNA
  • Transcription and translation occur simultaneously in bacteria as ribosomes begin translating an mRNA before its synthesis has been completed.

Figure 7.14

slide13
A bacterial mRNA may be polycistronic in having several coding regions that represent different genes.

Figure 7.15

7 8 eukaryotic mrna is modified during or after its transcription
7.8 Eukaryotic mRNA Is Modified During or after Its Transcription
  • A eukaryotic mRNA transcript is modified in the nucleus during or shortly after transcription.
slide15
The modifications include the addition of:
    • a methylated cap at the 5′ end
    • a sequence of poly(A) at the 3′ end

Figure 7.16

slide16
The mRNA is exported from the nucleus to the cytoplasm only after all modifications have been completed.

Figure 7.17

7 9 the 5 end of eukaryotic mrna is capped
7.9 The 5′ End of Eukaryotic mRNA Is Capped
  • A 5′ cap is formed by adding a G to the terminal base of the transcript via a 5′–5′ link.
    • 1-3 methyl groups are added to the base or ribose of the new terminal guanosine.

Figure 7.18

7 10 the 3 terminus is polyadenylated
7.10 The 3′ Terminus Is Polyadenylated
  • A length of poly(A) ∼200 nucleotides long is added to a nuclear transcript after transcription.
  • The poly(A) is bound by a specific protein (PABP).
  • The poly(A) stabilizes the mRNA against degradation.
7 11 bacterial mrna degradation involves multiple enzymes
7.11 Bacterial mRNA Degradation Involves Multiple Enzymes
  • The overall direction of degradation of bacterial mRNA is 5′–3′.
  • Degradation results from the combination of:
    • endonucleolytic cleavages
    • followed by exonucleolytic degradation of the fragment from 3′→5′

Figure 7.20

7 12 mrna stability depends on its structure and sequence
7.12 mRNA Stability Depends on Its Structure and Sequence
  • The modifications at both ends of mRNA protect it against degradation by exonucleases.
  • Specific sequences within an mRNA may have stabilizing or destabilizing effects.
  • Destabilization may be triggered by loss of poly(A).

Figure 7.21

7 13 mrna degradation involves multiple activities
7.13 mRNA Degradation Involves Multiple Activities
  • Degradation of yeast mRNA requires removal of the 5′ cap and the 3′ poly(A).
  • One yeast pathway involves exonucleolytic degradation from 5′→3′.

Figure 7.24

slide22
Another yeast pathway uses a complex of several exonucleases that work in the 3′→5′ direction.
  • The deadenylase of animal cells may bind directly to the 5′ cap.

Figure 7.25

7 14 nonsense mutations trigger a surveillance system
7.14 Nonsense Mutations Trigger a Surveillance System
  • Nonsense mutations cause mRNA to be degraded.
  • Genes coding for the degradation system have been found in yeast and worms.

Figure 7.26

7 15 eukaryotic rnas are transported
7.15 Eukaryotic RNAs Are Transported
  • RNA is transported through a membrane as a ribonucleoprotein particle.
  • All eukaryotic RNAs that function in the cytoplasm must be exported from the nucleus.
slide25
tRNAs and the RNA component of a ribonuclease are imported into mitochondria.
  • mRNAs can travel long distances between plant cells.

Figure 7.28

7 16 mrna can be specifically localized
7.16 mRNA Can Be Specifically Localized
  • Yeast Ash1 mRNA forms a ribonucleoprotein that binds to a myosin motor.

Figure 7.29

slide27
A motor transports it along actin filaments into the daughter bud.
  • It is anchored and translated in the bud, so that the protein is found only in the bud.

Figure 7.30