1 / 33

( + )

( + ). Team Members. Undergraduate Students Aman Arya , Biology (Waterloo) Joseph Chan , Molecular Genetics (Toronto) Hannah Fong , Immunology / Chemistry (Toronto) Lutz Kummer , visiting Biology (Waterloo) Emanuel Nazareth , Biomedical Engineering (Toronto)

kasa
Download Presentation

( + )

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ( + )

  2. Team Members Undergraduate Students Aman Arya, Biology (Waterloo) Joseph Chan, Molecular Genetics (Toronto) Hannah Fong, Immunology / Chemistry (Toronto) Lutz Kummer, visiting Biology (Waterloo) Emanuel Nazareth, Biomedical Engineering (Toronto) Martina Spranger, visiting Biology (Waterloo) Max Wong, Biomedical Engineering (Toronto) Graduate Students Seema Nagaraj, IBBME and Dept. of Electrical Engineering (Toronto) Matt Scott, Dept. of Applied Mathematics (Waterloo) Cindy Ying Yin Yip, IBBME (Toronto) Technical Support Andrew Hessel, Lab Manager, Mak Lab, University Health Network Faculty Advisors Prof. Stephen Davies, IBBME and Dept. of Electrical Engineering (Toronto) Prof. Brian Ingalls, Dept. of Applied Mathematics (Waterloo)

  3. 32 34 36 38 40 42 Temperature (°C) Two Designs Cell See Us Temperature Sensor Bacterial Etch-A-Sketch

  4. Cell See Us Tuneable Temperature Sensor: Design

  5. Gfp I12007 CI LacI Rfp R0010 R0011 Temperature Sensitive Dimerization

  6. Gfp I12007 CI LacI Rfp R0010 R0011 Temperature Sensitive Dimerization

  7. R0010 R0011 Temperature Sensitive Dimerization I12007

  8. R0010 R0011 Temperature Sensitive Dimerization I12007

  9. R0010 R0011 Temperature Sensitive Dimerization I12007

  10. Cell See Us Tuneable Temperature Sensor

  11. T1 T2 T3 Cell See Us Tuneable Temperature Sensor

  12. T1 T2 T3 Cell See Us Tuneable Temperature Sensor

  13. T1 T2 T3 Cell See Us Tuneable Temperature Sensor

  14. T1 T2 T3 Cell See Us Tuneable Temperature Sensor Transcription rate at T1 Transcription rate at T2

  15. fp2 fp1 lacI CI Rfp 40000 nM 30000 Gfp 20000 10000 34 36 38 40 42 32 34 36 38 40 42 Temperature (°C) Temperature (°C) Modeling Results Increase in temperature decreases the repression by lacI

  16. Bacterial Etch-A-Sketch: Design

  17. Bacterial Etch-A-Sketch Write/Draw with Pipette filled with Lactose Design will glow green

  18. Bacterial Etch-A-Sketch Erase using pipette filled with Tetracyclin Picture will cease to glow green

  19. Bacterial Etch-A-Sketch

  20. LacZ (Metabolizes Lactose) LacI Gfp TetR R0011 R0040 Lactose Binds with LacI Lactose Inactive Repressor

  21. LacZ (Metabolizes Lactose) TetR R0011 R0040 Lactose Binds with LacI Lactose Inactive Repressor

  22. LacZ (Metabolizes Lactose) TetR R0011 R0040 Tetracyclin Binds with TetR aTc Inactive Repressor

  23. LacZ (Metabolizes Lactose) R0011 R0040 Tetracyclin Binds with TetR aTc Inactive Repressor

  24. LacZ (Metabolizes Lactose) R0011 R0040 LacZ metabolizes Lactose

  25. LacZ (Metabolizes Lactose) R0011 R0040 LacZ metabolizes Lactose

  26. LacZ (Metabolizes Lactose) LacI Gfp TetR R0011 R0040 LacZ metabolizes Lactose

  27. Project Plan and Execution

  28. Initial Plan • May – June • Brainstorm and finalize design • July – August • Obtain constructs and test • September – October • Make revisions, fine tune model parameters, obtain and test new constructs

  29. Actual Execution • May – August • Brainstorm designs • September • Found out we needed to prepare constructs ourselves • Reconfigure designs to use available parts • Recruitment of volunteers with wet lab experience • October – Early Nov. • Undergraduates began assembly • Coincided with peak of undergrad midterm season

  30. Parts Made BBa_J11042 BBa_J11051 B0031+ C0056R0040+ E0433 And possibly: BBa_J11052 R0011+ E0422

  31. Lessons for Next Year • Larger team • Schedule undergraduate lab time more effectively • Prepare team for assembly

  32. Questions?

More Related