rotating vessel wall for the adhesion of cardiac cells n.
Download
Skip this Video
Download Presentation
Rotating Vessel Wall For the Adhesion of Cardiac Cells

Loading in 2 Seconds...

play fullscreen
1 / 11

Rotating Vessel Wall For the Adhesion of Cardiac Cells - PowerPoint PPT Presentation


  • 96 Views
  • Uploaded on

Rotating Vessel Wall For the Adhesion of Cardiac Cells. [Roli Kumar] [Advisor: Dr. Marc Lin]. Overview of Last Presentation. Importance of building a rotating vessel for replacement of damaged heart tissue and drug therapy research

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Rotating Vessel Wall For the Adhesion of Cardiac Cells' - karis


Download Now An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
overview of last presentation
Overview of Last Presentation
  • Importance of building a rotating vessel for replacement of damaged heart tissue and drug therapy research
  • objective was to develop 3-D cardiac tissue from a monolayer of cardiac cells
  • Basic design specifications of the rotating vessel
apparatus set up
Apparatus Set-Up

Suction cups

Plastic Rod

Plastic/metal

Rotating vessel

2.5cm

1.5cm

1.25cm

Magnetic Stirrer

how many cells are needed
How many cells are needed?
  • 1.6X 10^5 cells/cm^2
  • surface area= SA of cylinder+SA half circle
  • SA=2(pi)rh +.5(4(pi)r^2)
  • No. of cells= SA sphere X (cell/cm^2)
  • No. of cells= 3,455,200 cardiac cells
cardiomyocytes
Cardiomyocytes
  • Chick embryos
  • Chicks will be 7-8 days old
    • higher disassociation rate with younger chicks
    • older chicks produce low yield in disassociated cells
rotating vessel
Rotating Vessel
  • Metal ball
    • collagen will not bind
  • Plastic ball
    • collagen will bind
      • rat collagen
    • will not rotate with magnetic stirrer
      • aluminum foil
design specifications
Design Specifications
  • End-over end mixing
    • measurements at various speeds
    • problem: too fast, too slow
  • Solid body rotation
    • suspending cells in a liquid medium
    • inject the cells in
      • minimizing air bubbles
design specifications1
Design Specifications
  • Turbulence
    • suction cups
    • tubes connected
  • Aeration
    • medium usually contain bicarbonate
      • produces CO2
    • HEPES
      • not a bicarbonate buffer
work completed
Work Completed
  • Learned procedure for Cardiac Myocyte Isolation Protocol
    • cells were infected with bacteria
current work
Current Work
  • Repeat procedure using the ventricular portion of the heart
    • add penicillin to prevent bacterial growth
  • Sanitize rotating vessel
  • Put apparatus together
future plan of action
Future Plan of Action
  • Measure the speed of the rotating vessel
  • Model the forces applied on the cell