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Formación de EtOH a partir de Glicerol. Escenarios para el Biodiesel. 17000 Barriles = 969000 L de Biodiesel = 96900 L de glicerol. Problemâtica del Glicerol.

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escenarios para el biodiesel
Escenariospara el Biodiesel

17000 Barriles = 969000 L de Biodiesel = 96900 L de glicerol

problem tica del glicerol
Problemâtica del Glicerol
  • El glicerolcomosubproducto de la industriaimplicaunagranfuente de contaminaciôn y alto costo de procesoparadesecho del mismo sin obteneralgunarentabilidadporsuprocesado.
  • La granoferta y la bajademandahacen del glicerolunamateria prima de muybajoprecio.
ruta metab lica
Ruta Metabólica

Única Fuente de C

Anaerobiosis

EtOH

glicerol quinasa
Glicerol Quinasa
  • 4 Subunidades
  • Km= 10mM
  • pH = 9.5 ----- Km = 50 mM
  • 0°C --------Km = 25 mM
  • Kd= 10 mM
  • 3.7 mol glicerol/ mol enzima
glicerol quinasa inhibici n
Glicerol Quinasa- Inhibición
  • Fructosa 1,6 bifosfato
              • Ki= 0.5 mM
              • No competitivo
  • Glucosa
  • ATP
  • Mg 2+
  • Glicerol 3P
              • Ki= 0.5 mM

Inhibidores Leves

estructura primaria
Estructura Primaria
  • 502 aa
  • Masa Molar: 56106
  • Ac Glutámico
  • -Carboxil terminal
  • Treonina
  • -Amino terminal
microorganismos posibles
Microorganismos Posibles
  • Escherichia coli
  • Zymomonasmobilis
  • Bacillussubtilis
  • Saccharomycescerevisiae
bibliograf a
Bibliografía
  • Catalytic and Allosteric Properties of Glycerol Kinase from Escherichia coli*JEREMY W. THORNER AND HENRY PAULU Department of Biological Chemistry, Harvard Medical School, THE JOURNAL OF BIOLOGICAL CHEMISTRY.
  • CRECIMIENTO Y FORMACIÓN DE PRODUCTOS EN CULTIVOS AERÓBICOS Y ANAERÓBICOS DE Bacillussubtilis,CLAUDIA IBETH HERNÁNDEZ BUSTOS IBT
  • Tecnología de la fermentación del etanol - mobilis de Zymomonas P. Gunasekaran y K. ChandraRajDepartamento de la tecnología microbiana, escuela de ciencias biológicas, universidad de MaduraiKamaraj, Madurai
  • Theglycerolkinase )GUT1= gene of Saccharomycescerevisiae; cloning and characterization. P Pavlik, M Simon, T Shchuster, H. Ruis
  • http://www.eimaep.org/pdfs/rutas/24.pdf
  • Escherichia coli GlycerolKinase CLONING AND SEQUENCING OF THE glpK GENE AND THE PRIMARY STRUCTURE OF THE ENZYMEDonald W. PettigrewS, Din-Pow Ma#, Charles A. Conrad#, and James R. JohnsonnDepartment of Biochemistry and Biophysics and the $Department of Biology, Texas A&M University