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THAP11 和 ABRO1 的相互作用

THAP11 和 ABRO1 的相互作用. 2012-12-3 徐婧. 工作进展. G2 cells. 过表达 THAP11 下调内源 Abro1 表达. MYC(800 ng ): + + + + - - - - M-THAP11(800 ng ): - - - - + + + +

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THAP11 和 ABRO1 的相互作用

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  1. THAP11和ABRO1的相互作用 2012-12-3 徐婧

  2. 工作进展 G2 cells 过表达THAP11下调内源Abro1表达 MYC(800ng): + + + + - - - - M-THAP11(800ng): - - - - + + + + CHX(h): 0 8 18 24 0 6 12 24 Abro1 Myc-THAP11 GAPDH MYC(1μg): + + + + - - - - M-THAP11(1μg): - - - - + + + + CHX(h): 0 8 18 24 0 6 12 24 Abro1 Myc-THAP11 GAPDH

  3. 工作进展 G2 cells 过表达THAP11下调内源Abro1表达 MYC(800ng): + + + + - - - - M-THAP11(800ng): - - - - + + + + FAbro1(200ng): + + + + + + + + CHX(h): 0 8 18 24 0 6 12 24 Abro1 myc-THAP11 GAPDH

  4. 外源THAP11和ABRO1间相互作用 M-THAP11 F-ABRO1 Myc Flag IB:ABRO1 IB:THAP11 IP:flag IP:Myc IB:ABRO1 IB:THAP11 IB:ABRO1 IB:THAP11 Input Input IB:ABRO1 IB:THAP11

  5. 下一步工作计划 • 确定ABRO1-THAP11内源相互作用及复合体形成； • RT-PCR确定ABRO1调控THAP11转录； • RT-PCR确定THAP11调控ABRO1转录。

  6. 工作计划 1.确定ABRO1稳定THAP11； 2.Real-Time PCR检测ABRO1对THAP11在转录水平上调控； 3.外源和内源Co-IP实验研究THAP11-ABRO1相互作用； 4. 免疫荧光检测ABRO1和THAP11亚细胞共定位； 5. ABRO1通过THAP11抑制集落形成（构建敲低THAP11过表达ABRO1的稳定细胞株）。 6.THAP11是否影响ABRO1参与DNA损伤应急反应（DDR）,THAP11是否影响ABRO1的核质穿梭？？

  7. 疑问 • ABRO1稳定THAP11, （ABRO1通过稳定p53抑制增殖，THAP11通过抑制C-myc转录抑制增殖），ABRO1是否还通过THAP11来抑制细胞增殖？敲低THAP11过表达ABRO1是否还能抑制增殖？？ • THAP11促进ABRO1的降解？？？

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