Second year Lab Medicine (Females)
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Second year Lab Medicine (Females) 1433- 1434 A.H. / 2012- 2013 Microbiology Practical (Course I). By: Hibah A. W. Abusulaiman. Once upon a time…. Please don’t sleep. Fashionable friends. Abrar’s …. Sara started the preparation early. She got a luxurious manicure and padicure plus….

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By hibah a w abusulaiman

Second year Lab Medicine (Females)

1433- 1434 A.H. / 2012- 2013

Microbiology Practical (Course I)

  • By:

    Hibah A. W. Abusulaiman


Once upon a time

Once upon a time…

Please don’t sleep







Lubna started the preparation 2 days before the wedding
Lubna started the preparation 2 days before the wedding





After 45 days lubna was suffering from
After 45 days, Lubna was suffering from…



After discussion with the doctor she knew it s because of the poor hygienic manicure and pedicure
After discussion with the Doctor, she knew it’s because of the poor hygienic manicure and pedicure


By hibah a w abusulaiman

Good hygiene the poor hygienic manicure and pedicure

Poor hygiene


Destruction of microorganisms

Destruction of Microorganisms the poor hygienic manicure and pedicure


So do you think we are in a war with
So, do you think we are in a war with…?! the poor hygienic manicure and pedicure


Simply because they are the agents of

Simply because they are the agents of… the poor hygienic manicure and pedicure

Contamination, infection and decay


Early civilization
Early civilization the poor hygienic manicure and pedicure


In the 1800s

In the 1800s the poor hygienic manicure and pedicure

Aseptic techniques invented to prevent contamination of surgical wounds


Many organizations classified infection control systems and substances into
Many organizations classified infection control systems and substances into:

  • Physical methods.

  • Chemical methods.

Referred broadly as Germicides


Germicidal systems fall into three categories
Germicidal systems fall into three categories: substances into

  • Decontamination.

  • Disinfection.

  • Sterilization.


1 decontamination
1. Decontamination: substances into

  • Reduction of pathogenic microorganisms to be safe to handle without protective attire.

  • The Lowest level of control.

  • Includes physical cleaning with soap and detergents and removing of most organic and inorganic materials.

  • It is the critical step toward disinfection and steilisation.


2 disinfection
2. Disinfection: substances into

  • Disinfectants are liquid chemical agents that kill targeted pathogens but not spores . It can also be solid or gaseous.

  • It is the next level of control.


3 sterilization
3. Sterilization substances into

  • The complete elimination of viable organisms including spores.

  • The highest level of control


Factors influencing disinfection
Factors influencing disinfection substances into

  • Number of microorganisms.

  • Species and type of microorganism.

  • Temperature.


Physical antimicrobial agents
Physical Antimicrobial Agents substances into

  • Physical environmental agents can destroy large concentrations of microorganisms in lab cultures or a clinical specimen.



Radiation
Radiation substances into

UV Light

To inactivate microorganisms on surfaces


Filtration
Filtration substances into

  • Filtration is used to remove bacteria, fungi and their endospores from air or solutions.


Osmotic pressure
Osmotic Pressure substances into

  • Extremely hypertonic conditions can cause plasmolysis.


Incineration
Incineration substances into


Autoclave moist heat steam sterilization 121 o c for 15 min
Autoclave substances intoMoist heat- Steam sterilization121OC for 15 min


Dry heat 160 o c for 2 hours or 170 o c for an hour
Dry heat substances into160OC for 2 hours OR 170OC for an hour


Chemical antimicrobial agents
Chemical Antimicrobial Agents substances into

  • In considering their application to patient care, two general classes:

    • Those destroying pathogenic microorganisms in the environment (disinfectants) or on skin (antiseptics).

    • Those administered to patients for treatment of infectious diseases (antimicrobial agents, often called antibiotics).


Desinfectants
Desinfectants substances into


Evaluating a disinfectant
Evaluating a disinfectant substances into

  • Direct spray method.

  • Testing the quality of disinfectant from different brands.


By hibah a w abusulaiman

Testing Disinfectant from Different Brands substances into

0.5 mL

5 mLDettol

5 mL Clorox

5 mL DAC

5 mLGento

5 mL Bacterial Suspension in Nutrient Broth (Control)


By hibah a w abusulaiman

Testing Disinfectant from Different Brands substances into

Water Bath

Bacteria in Clorox

Bacteria in Dettol

Bacteria in DAC

Bacteria in Gento

Bacterial Suspension in Nutrient Broth (Control)


By hibah a w abusulaiman

Testing Disinfectant from Different Brands substances into

2 Loopfulls

48 hrs

Incubation

in the 37O C

Water Bath

After 5min

in Water Bath

After 10 min in

Water Bath

After 20 min in

Water Bath

Bacterial Suspension in Nutrient Broth (Control)



Objectives
Objectives substances into

  • Some infection control terms.

  • Factors influencing disinfectants.

  • Physical destruction of microorganisms.

  • Chemical destruction of microorganisms.

  • Methods of evaluating disinfectants.


Thank you for listening any questions
Thank you for listening substances intoAny questions?!


Thank you for paying attention

Thank you for paying attention substances into

Any questions?!