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TTTATAAAATTATAC TGTT

Supplementary Table 2. Junctional sequences observed in survivals of SLY19 and its mutant derivatives. The original sequences that constitute noncomplementary single-stranded ends are underlined. Microhomologies with overlapping sequencesare shown

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TTTATAAAATTATAC TGTT

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  1. Supplementary Table 2. Junctional sequences observed in survivals of SLY19 and its mutant derivatives The original sequences that constitute noncomplementary single-stranded ends are underlined. Microhomologies with overlapping sequencesare shown in boldface. The deletions are shown in parentheses. Negative numbers in the “Modifications” column indicate the number of nucleotides deleted in either side of the DSBs.In cases of imperfect overlap, mismatches with several matching bases on either side were only considered as microhomologies. The number of overlapping bases is shown to indicate the total number of overlapping sequences (in parentheses) and the identical sequences among them No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATA(CTGTT) AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAGG -5/0 1 1 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 4 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 1 TTTATAAAATTATACTG(TT) (AACAGTATAATTTTATAAACC) CTGGTTTTGGTTTTGTAGAG -2/-21 3 1 TTTATAAAATTATA(CTGTT) (AACAG) TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 2 SLY19 (wt) TTTATAAAATTATA(CTGTT) (AACAGTATAATT) TTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-12 10(12) 2 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 29 TTTATAAAATTAT(ACTGTT) (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG -6/-53 9(10) 5 TTTATAAAAT(TATACTGTT) (AACAGTATAA--------GTCTATGTATTTG) TATAAAATAT -9/-293 8 1

  2. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 2 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 6 TTTATAAAATTATACTG(TT) (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -2/-2 2(3) 1 SLY19 rad51∆ TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 5 TTTATAAAATTAT(ACTGTT) (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG -6/-53 9(10) 5 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 13 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 20 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 26 SLY19 sae2∆ TTTATAAAATTATAC(TGTT) AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/0 2 1 TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 6 (AACAGTATAA)TTTTATAAACCCTGGTTTTGGTTTTGTAGAG TTTATAAAATTATACTGTT 0/-10 2 1

  3. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 24 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 17 TTTATAAAATTATACTG(TT) (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -2/-2 0 2 SLY19 tel1∆ TTTATAAAATTATAC(TGTT) (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-4 0 1 TTTATAAAATTATACT(GTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -3/-4 1 1 TTTATAAAATTATACTGTT (AACAGTATAA)TTTTATAAACCCTGGTTTTGGTTTTGTAGAG 0/-10 2 1 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 2 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 4 SLY19 msh2∆ TTTATAAAATTATACTG(TT) (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -2/-2 0 1 TTTATAAAATTATA(CTGTT) (A)ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-1 1 64 TTTATAAAATTAT(ACTGTT) (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG -6/-53 9(10) 5 TTTAT(AAAATTATACTGTT) (AACAGTATAATTTT)ATAAACCCTGGTTTTGGTTTTGTAGAG -14/-14 2 5 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 6

  4. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 11 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 12 TTTATAAAATTATACTGTT (AACAGTAT)AATTTTATAAACCCTGGTTTTGGTTTTGTAGAG 0/-8 0 1 SLY19 srs2∆ TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 5 TTTATAAAATTATACTG(TT) (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -2/-2 0 2 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 4 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 17 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 22 TTTATAAAATTATACTG(TT) (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -2/-2 0 2 TTTATAAAATTATACT(GTT) (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -3/-4 1 1 SLY19 rad10∆ TTTATAAAATTATACTGTT (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG 0/-5 1 1 TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-4 0 1 TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 1 TTTAT(AAAATTATACTGTT) (AACAGTATAATTTT)ATAAACCCTGGTTTTGGTTTTGTAGAG -14/-14 2 1 TTTATAAAATTATA(CTGTT) (AACAGTATAATT) TTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-12 8(10) 2

  5. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 3 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 5 TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 2 SLY19 rad27∆ TTTATAAAATTAT(ACTGTT) (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG -6/-53 9(10) 1 TTTAT(AAAATTATACTGTT) (AACAGTATAATTTT)ATAAACCCTGGTTTTGGTTTTGTAGAG -14/-14 10(12) 2 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 13 TTTATAAAATT(ATACTGTT) AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -8/0 2(4) 1 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 4 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 5 SLY19 pol4∆ TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 36 TTTATAAAATTATA(CTGTT) (AACAGTATAATT) TTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-12 8(10) 2

  6. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 2 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 4 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 27 SLY19 exo1∆ TTTATAAA(ATTATACTGTT) (AACAGTATAAT)TTTATAAACCCTGGTTTTGGTTTTGTAGAG -11/-11 8 1 TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 1 TTTATAAAATTATACTG(TT) (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -2/-2 0 2 TTTATAAAATTATAC(TGTT) (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-4 0 1 TTTAT(AAAATTATACTGTT) (AACAGTATAATTTT)ATAAACCCTGGTTTTGGTTTTGTAGAG -14/-14 10(12) 1 TTTATAAAATTA(TACTGTT) AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -7/0 1 1 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 2 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 3 SLY19 msh3∆ TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 1 TTTATAAAATTATAC(TGTT) (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-4 0 1 TTTATAAAATTAT(ACTGTT) (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG -6/-53 9(10) 6 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 9

  7. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 9 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 12 TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 3 SLY19 mre11∆ pmre11- H125N TTTATAAAATTATACTG(TT) (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -2/-2 0 1 TTTATAAAATTAT(ACTGTT) (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG -6/-53 0 1 TTTATAAAATTAT(ACTGTT) AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -6/0 0 1 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 7 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 7 SLY19 Gal:pol4ΔBr TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 17 TTTATAAAATTATAC(TGTT) (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-4 0 1 TTTATAAAATTATACTGTT (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG 0/-5 1 6 TTTAT(AAAATTATACTGTT) (AACAGTATAATTTT)ATAAACCCTGGTTTTGGTTTTGTAGAG -14/-14 10(12) 1 TTTATAAA(ATTATACTGTT) (AACAGTATAAT)TTTATAAACCCTGGTTTTGGTTTTGTAGAG -11/-11 8 1 TTTATAAAATTATACTG(TT) (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -2/-2 0 2

  8. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 18 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 11 TTTATAAAATTATAC(TGTT) (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-4 0 3 SLY19 pol4∆ Gal:pol4-D367E TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 7 TTTATAAAATTATAC(TGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-5 0 1 TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 4 TTTATAAAATTATA(CTGTT) (AACAGTATAATT) TTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-12 10(12) 2 TTTATAAAATT(ATACTGTT) AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -8/0 2(4) 1 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 4 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 3 SLY19 sgs1∆ TTTATAAAATTATA(CTGTT) (AACAGTATAATT) TTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-12 10(12) 3 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 17

  9. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 8 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 6 TTTATAAAATTATACTG(TT) (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -2/-2 2(3) 2 SLY19 pol32∆ TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 1 TTTATAAAATTATACTGTT (AACAGTATAA)TTTTATAAACCCTGGTTTTGGTTTTGTAGAG 0/-10 2(4) 1 TTTATAAAATTATACTGTT (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG 0/-5 7(9) 1 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 2 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 5 SLY19 rad59∆ TTTATAAAATTAT(ACTGTT) (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG -6/-53 9(10) 3 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 7 TTTATAAAATTATA(CTGTT) (AACAGTATAATT) TTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-12 10(12) 2 TTTATAAAATTATAC(TGTT) (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-4 0 1

  10. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 6 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 4 T(TTATAAAATTATACTGTT) (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -18/-4 2 1 SLY19 pol32∆ pol4∆ TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 1 TTTATAAAATTATACTGTT (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG 0/-5 1 1 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 1 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 14 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 10 SLY19 rev3∆ TTTATAAAATTATAC(TGTT) AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/0 2 1 TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 6 TTTATAAAATTATACTGTT (AACAGTATAA)TTTTATAAACCCTGGTTTTGGTTTTGTAGAG 0/-10 2 1 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 3

  11. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 13 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 3 TTTATAAAATTATA(CTGTT) (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-5 4 1 SLY19 rad30∆ TTTATAAAATT(ATACTGTT) AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -8/0 2(4) 1 TTTATAAAATTATAC(TGTT) (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-4 0 1 TTTATAAAATTAT(ACTGTT) (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG -6/-53 9(10) 4 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 1 TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 5 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 6 SLY19 rev3∆ rad30∆ TTTATAAAATTAT(ACTGTT) (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG -6/-53 9(10) 8 TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 2 TTTATAAAATTATACTGTT (AACAGTATAA)TTTTATAAACCCTGGTTTTGGTTTTGTAGAG 0/-10 2 1 TTTATAAAATT(ATACTGTT) AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -8/0 2(4) 2

  12. No. of overlapping bases No. of events Modifications Junctonal sequence Strain GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA Parental TTTATAAAATTATACTGTT AAATATTTTAATATG TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT TTTATAAAATTATAC(TGTT) (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -4/-1 2 2 TTTATAAAATTATACTGT(T) (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG -1/-4 2 5 SLY19 nej1∆ TTTATAAAATTATACTG(TT) (AACAGTATAA---GAGTGGTTGACGAAT AATTATG) CTGAAG -2/-60 9(10) 16 TTTATAAAATTATA(CTGTT) (AACAGTATAATT) TTATAAACCCTGGTTTTGGTTTTGTAGAG -5/-12 8(10) 3

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