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Enzyme Function Experiment: pH, Temperature & Substrate Levels

Explore how pH, temperature, and substrate levels impact enzyme function. Design experiments, compare graphs, and learn enzyme mechanisms. Visit http://www.lewport.wnyric.org/jwanamaker/animations/Enzyme%20activity.html to enhance your understanding.

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Enzyme Function Experiment: pH, Temperature & Substrate Levels

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    1. When you get here: Start labeling tubes - Label 5 tubes: C1a – C5a Label 5 tubes: C1b – C5b Label 5 tubes: C1c – C5c Label 5 tubes: C1d – C5c Begin heating beakers of water for the Benedicts test

    2. Objectives Explain how pH, temperature and substrate levels effect enzyme function Design an experiment that would show effectiveness of an enzyme Compare and contrast standard, optimum and time course graphs

    3. Enzymes: function Catalyze reactions by lowering activation energy Accelerate reactions by orienting the substrate http://www.lewport.wnyric.org/jwanamaker/animations/Enzyme%20activity.html

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