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Magnetic Tweezer System Development. Probing mechanical properties across multiple scales. Jason Sherfey Senior BME, Vanderbilt University. Advisor: Dr. Franz Baudenbacher. Force. 1 nN. 3. 2. T=0 s. displacement [ m m]. 1. 0. 0. 1. 2. 3. Time [s]. F. T=1.5 s.

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Magnetic tweezer system development

Magnetic Tweezer System Development

Probing mechanical properties across multiple scales

Jason Sherfey

Senior BME, Vanderbilt University

Advisor: Dr. Franz Baudenbacher


Magnetic tweezer system development

Force

1 nN

3

2

T=0 s

displacement [mm]

1

0

0

1

2

3

Time [s]

F

T=1.5 s

Force displacement measurements on magnetic beads linked to the cell surface through E-Cadherin

Fit to Mechanical Analog

Extract Model parameter


Magnetic tweezer system development

Force-Displacement Curves MDCK cells

P120 Knockout

Wild-type





Magnetic tweezer system development

Viscosity (Pa-s-m)

p = 0.364

Mean

Standard Deviation

P120 Knockout MDCK

0.0038

0.0019

Wild-type MDCK

0.0044

0.0020

Elasticity (Pa-m)

p = 0.0028

Mean

Standard Deviation

P120 Knockout MDCK

0.0118

0.0106

Wild-type MDCK

0.0217

0.0088

Relaxation Time (s)

p = 0.0010

Mean

Standard Deviation

P120 Knockout MDCK

0.0725

0.0006

Wild-type MDCK

0.0421

0.0006

No significant difference in WT & KO Viscosities

WT Elasticity is significantly larger than KO

WT Relaxation Time is significantly faster than KO

N = 10 cells


Conclusions
Conclusions

  • The stiffness and relaxation time constants are significantly different in p120 knockout and wild-type MDCK cells.

  • 2. The stiffness decreases & relaxation time slows down when p120 expression is reduced in MDCK cells.


Strain hardening
Strain Hardening

Stiffness (Pa-m)

  • The stiffness of the adhesion protein linker system increases when stress is repeatedly applied.