evolution of enterovirus 71
Download
Skip this Video
Download Presentation
Evolution of Enterovirus 71

Loading in 2 Seconds...

play fullscreen
1 / 24

Evolution of Enterovirus 71 - PowerPoint PPT Presentation


  • 342 Views
  • Uploaded on

Evolution of Enterovirus 71. Jen-Ren Wang, Ph. D. ( 王貞仁 ) Dept. of Medical Laboratory Science and Biotechnology, National Cheng Kung University NHRI Tainan Virology Laboratory for Diagnosis and Research Taiwan CDC Virology Contract Laboratory. Outline. Enterovirus surveillance in Taiwan

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Evolution of Enterovirus 71' - judith


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
evolution of enterovirus 71
Evolution of Enterovirus 71

Jen-Ren Wang, Ph. D. (王貞仁)

  • Dept. of Medical Laboratory Science and Biotechnology, National Cheng Kung University
  • NHRI Tainan Virology Laboratory for Diagnosis and Research
  • Taiwan CDC Virology Contract Laboratory
slide2
Outline
  • Enterovirus surveillance in Taiwan
  • Molecular analysis of EV71
  • Virulent Gene Analysis of Enterovirus 71 and Disease Association
slide3
Taiwan CDC and Virology Contract Laboratories

Taipei Veterans General Hospital

Chang Gung Memorial Hospital, Linkou

Tri-Service General Hospital

Taiwan University Hospital

China Medical University Hospital

Buddhist Tzu Chi General Hospital

Chang Gung Memorial Hospital, Kaoshiung

Cheng Kung University Hospital

Kaoshiung Medical University Hospital

CDC, Taiwan

Taichung Veterans General Hospital

Chunghua Christian Hospital

Kaoshiung Veterans General Hospital

slide5
Enteroviruses isolated in contract labs-I

2000 (n=1310)2001 (n=2114) 2002 (n=1839)

Rank Serotype % Serotype % Serotype %

1 Untyped 44.4 Untyped 43.8 Untyped 45.9

2 EV 71 18.2CA 16 15.5CA 16 16.2

3 CA 16 17.1EV 71 15.0 E 6 10.2

4 CB 3 4.9E 30 12.1CB 5 9.4

5 E 9 4.6E 6 5.4 EV 71 7.9

6 CB 4 4.0 P 3.8CA 24 3.3

7 E 4 1.3 CB 4 1.6 CB 2 2.3

8 P 0.8 CA 24 0.8 P 1.6

9 CA 4 0.7 CA 9 0.5 E 9 0.8

10 E 3 0.7 CB 5 0.5 E 4 0.5

11 CB 1 0.6 CA 10 0.3 CB 4 0.5

12 CA 24 0.5 CA 6 0.1 CA 10 0.4

13 CA 9 0.3CB 1 0.1 CA 4 0.3

14 CA 2 0.3 CB 3 0.1 E 11 0.2

15 most frequent

serotypes 98.4 99.6 99.5

slide6
Enteroviruses isolated in contract labs-II

2003 (n=2066) 2004 (n=2389) 2005 (n=2031)

Rank Serotype % Serotype % Serotype %

1 CA 16 35.8 CA 4 23.8 CB 3 30.6

2 Untyped17.7CA 10 22.7CA 16 25.5

3 EV 71 13.0CB 4 16.5EV 71 13.9

4 E 9 7.5Untyped 9.8CA 6 6.6

5 E 11 6.2EV 71 8.5 CA 5 5.9

6 CA 2 4.7 CA 6 2.6Untyped 5.3

7 CA 6 3.9 CA 9 2.6 CA 2 3.8

8 CA 5 3.2 CB 3 2.2 E 9 3.3

9 CB 2 2.0 CA 5 2.0 CB 2 1.4

10 CB 5 1.3 CA 2 1.8 CA 10 1.0

11 P 0.9 CA 16 1.7 P 0.9

12 CA 11 0.6 CB 2 1.2 CA 4 0.6

13 E 30 0.6E 25 1.0 CA 9 0.5

14 CA 10 0.5 E 11 0.9 CB 1 0.2

15 most frequent

serotypes 97.9 97.3 99.5

clinical presentations of ev71
Clinical presentations of EV71
  • Infection of EV71 can cause hand-foot-and-mouth disease (HFMD), herpangina, aseptic meningitis, acute flaccid paralysis (AFP), brainstem encephalitis, and pulmonary edema.
  • Several death-associated outbreaks of EV71 infections occurred in Taiwan from 1998 to 2005.
enterovirus 71 ev71
Enterovirus 71(EV71)

1975, Bulgaria

1978, Hungary

1997, Japan

1969, California

1998, Taiwan

1998, Singapore

1997, Malaysia

1999, Australia

Ho, M, et al. Engl J Med. 1999.

ev71 in west pacific region
EV71 in West Pacific region
  • Large EV71 epidemics were also reported in Malaysia, Japan, Singapore, Australia, China, Korea, India.
slide11
Outline
  • Enterovirus surveillance in Taiwan
  • Molecular analysis of EV71
  • Virulent Gene Analysis of Enterovirus 71 and Disease Association
slide12
1986

1998

1999

2000

2001

2002

2003

2004

2005

CA16

A

BrCr

7423-MS-87

72

237-TW-86

Phylogenetic analysis of EV71 isolates in Taiwan from 1986 and 1998-2005 based on VP1 region

Both genotypes were able to cause mild and severe diseases.

242-TW-86

99

99

N2838-TW-03

9-97-SHA89

99

5145-TW-98

236-TW-86

97

99

N3793-TW-01

99

N0991-TW-01

S0320-TW-02

81

B

N2443-TW-02

N3496-TW-02

5666-SIN-002209

99

91

5865-SIN-000009

99

N0781-TW-01

N1643-TW-00

H0148-TW-00

80

S0875-TW-99

S0942-TW-99

S0943-TW-99

99

M0167-TW-00

N0716-TW-00

M0201-TW-00

99

S18191-SAR-02

02363-Thai-02

93

1814-JAP-01

1585-JAP-01

99

99

1695-JAP-01

2086-TW-98

99

5746-TW-98

H0001-TW-98

H0358-TW-98

NCKU9822

6092-TW-98

99

H0139-TW-98

4643-TW-98

1245a-TW-98

4-SHZH-01

C

2779-JAP-02

N3340-TW-02

SHZH03

99

40-SHZH-02

03-SHZH-01

08-SHZH-01

99

62-SHZH-02

N2735-TW-04

S0584-TW-04

N2121-TW-05

99

N2472-TW-04

N1862-TW-05

N2490-TW-04

N1614-TW-04

N0003-TW-05

N3031-TW-04

N2411-TW-04

0.1

0.1

slide13
Phylogenetic tree for VP1 region of EV71 strain isolated in Yamagata, Japan, between 1998 and 2003 and reference strains.

Mizuta et al, J Clin Micro 43: 6171, 2005

slide15
Appearance of intratypic recombination of EV71
  • Recombination is a well-known phenomenon for enteroviruses.
  • The actual extent of recombination within circulating EV71 is not known.
slide17
1986

1998

1999

2000

2001

2002

2003

2004

2005

CA16

A

BrCr

7423-MS-87

72

237-TW-86

Phylogenetic analysis of EV71 isolates in Taiwan from 1986 and 1998-2005 based on VP1 region

242-TW-86

99

99

N2838-TW-03

9-97-SHA89

99

5145-TW-98

236-TW-86

97

99

N3793-TW-01

99

N0991-TW-01

S0320-TW-02

81

B

N2443-TW-02

N3496-TW-02

5666-SIN-002209

99

91

5865-SIN-000009

99

N0781-TW-01

N1643-TW-00

H0148-TW-00

80

S0875-TW-99

S0942-TW-99

S0943-TW-99

99

M0167-TW-00

N0716-TW-00

M0201-TW-00

99

S18191-SAR-02

02363-Thai-02

93

1814-JAP-01

1585-JAP-01

99

99

1695-JAP-01

2086-TW-98

99

5746-TW-98

H0001-TW-98

H0358-TW-98

NCKU9822

6092-TW-98

99

H0139-TW-98

4643-TW-98

1245a-TW-98

4-SHZH-01

C

2779-JAP-02

N3340-TW-02

SHZH03

99

40-SHZH-02

03-SHZH-01

08-SHZH-01

99

62-SHZH-02

N2735-TW-04

S0584-TW-04

N2121-TW-05

99

N2472-TW-04

N1862-TW-05

N2490-TW-04

N1614-TW-04

N0003-TW-05

N3031-TW-04

N2411-TW-04

0.1

0.1

slide18
CA16

A

BrCr

NCKU9822

6092-TW-98

2086-TW-98

5746-TW-98

H0001-TW-98

H0139-TW-98

H0358-TW-98

4643-TW-98

1245a-TW-98

SHZH03

99

C

N3340-TW-02

N0003-TW-05

N1862-TW-05

N2735-TW-04

S0584-TW-04

N2490-TW-04

N3031-TW-04

N2411-TW-04

N2472-TW-04

N1614-TW-04

N2121-TW-05

237-TW-86

99

242-TW-86

236-TW-86

7423-MS-87

N2838-TW-03

5145-TW-98

93

9-97-SHA89

99

N2443-TW-02

99

N0991-TW-01

N3793-TW-01

B

S0320-TW-02

N3496-TW02

89

5666-SIN-002209

5865-SIN-000009

98

N1643-TW-00

N0781-TW-01

91

M0201-TW-00

N0716-TW-00

M0167-TW-00

S0875-TW-99

H0148-TW-00

S0942-TW-99

S0943-TW-99

0.1

Phylogenetic analysis of EV71 isolates in Taiwan from 1986 and 1998-2005 based on 3D region

1986

1998

1999

2000

2001

2002

2003

2004

2005

slide19
CA16

A

BrCr

2086-TW-98

99

H0358-TW-98

H0139-TW-98

4643-TW-98

C

NCKU9822

1245a-TW-98

6092-TW-98

5746-TW-98

H0001-TW-98

N3340-TW-02

83

81

SHZH03

99

N0003-TW-05

N2121-TW-05

99

N1862-TW-05

B

S0584-TW-04

1986

N2735-TW-04

N2472-TW-04

1998

N2490-TW-04

1999

N2411-TW-04

99

2000

N3031-TW-04

N1614-TW-04

2001

7423-MS-87

242-TW-86

2002

99

236-TW-86

2003

237-TW-86

2004

9-97-SHA89

91

5145-TW-98

2005

N2838-TW-03

N0991-TW-01

N3793-TW-01

92

99

S0320-TW-02

B

N3496-TW-02

N2443-TW-02

N0781-TW-01

N1643-TW-00

5666-SIN-002209

5865-SIN-000009

M0201-TW-00

N0716-TW-00

S0942-TW-99

S0943-TW-99

S0875-TW-99

H0148-TW-00

M0167-TW-00

0.1

Phylogenetic analysis of EV71 isolates in Taiwan from 1986 and 1998-2005 based on 2B region

slide20
Year

T

otal EV71

EV71 analyzed

Genotype of EV71 tested based on five regions

isolated

in this study

B

C

Genotype of

Recombinant

no. (%)

no. (%)

no (%)

5'

-

UTR

VP1

VP4

-

VP2

2B

3D

1986

unknown

7

7

(100.0)

0

0

B

B

B

B

B

1998

132

10

2 (20.0)

0

B

B

B

B

B

8 (80.0)

C

C

C

C

C

1999

13

4

4

(100.0)

0

0

B

B

B

B

B

2000

171

15

15

(100.0)

0

0

B

B

B

B

B

2001

116

5

5

(10

0.0)

0

0

B

B

B

B

B

2002

25

8

7 (100.0)

0

B

B

B

B

B

1 (12.5)

C

C

C

B

C

2003

10

4

4

(100.0)

0

0

B

B

B

B

B

2004

32

12

0

0

12

(100.0)

C

C

C

B

C

2005

34

8

0

1 (12.5)

C

C

C

C

C

7 (87.5)

C

C

C

B

C

Recombination of EV71 from 1986 and 1998-2005

slide21
3B

5′UTR

3′UTR

VP2

VP3

VP4

2A

2C

3A

3C

3D

2B

VP4

Simplot compared N3340/TW/02 with EV71 reference strains

slide22
3B

5′UTR

3′UTR

VP2

VP3

VP4

2A

2C

3A

3C

3D

2B

VP4

Bootscan compared N3340/TW/02 with EV71 reference strains

slide23
Summary of EV71 recombination study
  • Intratypic recombination of EV71 among nature circulating isolates was found to be initiated in 2002 and became predominant in 2004 and 2005.
  • Recombinant points were located at the 3'-termini of 2A and 3C regions, suggesting that nonstructural genes recombined to produce new EV71 variants.
slide24
Implications of EV71 study
  • The results indicated that standard typing may produce misleading results or even sequencing of multiple partial regions of EV71 will not always be sufficient.
  • It remains to be determined what role of the emergence of this new EV71 recombinant plays (e.g. clinical significance, vaccine selection).
ad