Joshua Demeo , Yin Wong, Cuiwen He, and Yang Liu May 31, 2012. Background . Biofuels are currently produced from carbohydrates and lipids in feedstock Problems: The algae based schemes have limited efficiency due to feedstock cultures having to be starved
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Joshua Demeo, Yin Wong, Cuiwen He, and Yang Liu
May 31, 2012
Isobutanol synthesis pathway
Yield: 2.3% of theoretical yield
E. Coli can grow well in yeast extract or mixtures of 20 amino acids, but the utilization of amino acids is incomplete.
Identified by GC-MS and quantified by GC-FID
C2: Ethanol (3%)
C4: Isobutanol (50%)
C5: C5 alcohols (47%)
Pseudomonas can grow on the six left over AA: Lys, Tyr, Phe, Trp, Met and His, and convert them to 20 amino acids.
Algal biomass mixture includes C. vulgaris, P. purpureum, S.platensis and S.elongatus.
C. vulgaris- green algae
P. purpureum-red algae
S. platensis-green blue algae
Algae and bacteria were grew and collected, digested with protease and used as feedstock for biofuel production with YH83.
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