chapter 9 identification of saliva and misc biological fluids
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Forensic Biology by Richard Li

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Chapter 9: Identification of Saliva and Misc. Biological Fluids. Forensic Biology by Richard Li. Biological Characteristics. Human salivary glands produce 1.0-1.5 liters per day Mostly water Amylase- digests carbohydrates in the oral cavity Identification of amylase indicates saliva

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biological characteristics
Biological Characteristics
  • Human salivary glands produce 1.0-1.5 liters per day
  • Mostly water
  • Amylase- digests carbohydrates in the oral cavity
    • Identification of amylase indicates saliva
    • Enzymes that cleave polysaccarides that are composed of D-glucose units connected by (α1-4) linkages
      • Starch

Forensic Biology by Richard Li

biological characteristics1
Biological Characteristics
  • Starch
  • Amylose
  • Amylopectin
  • Two types of amylases:
    • β-amylase
      • Plant and bacterial sources
    • α-amylase
      • Human
      • Human salivary (HSA)
      • Human pancreatic (HPA)

Forensic Biology by Richard Li

presumptive assays
Presumptive Assays
  • Visual Examination
    • ALS (470nm), Microscopic
  • Starch-iodine assays
    • Iodine used to test for starch
    • Not specific
  • Colorimetric assays
    • Dye-labeled amylase substrates are employed
    • Phadebas test

Forensic Biology by Richard Li

confirmatory assays
Confirmatory Assays
  • Immunochromatographic Assays
    • RSID-Saliva
  • ELISA
    • Monoclonal HSA antibody
  • RNA-based assays
    • Certain genes are specifically expressed in certain types of cells

Forensic Biology by Richard Li

misc biological fluids
Misc. Biological Fluids
  • Vaginal secretions, Menstrual blood, Urine, Feces, Vomitus
    • Less frequently encountered
    • Can be probative in certain cases

Forensic Biology by Richard Li

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