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All variant calls (VCF file)

Ion Proton Sequencing of paired Normal-tumor sample. +. All variant calls (VCF file). Sequencing Depth (Coverage file). OncoSeek Bioinformatics tool (Annotation, filtering and reporting tool). Low specificity variant filter Filters sequencing artifacts :

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All variant calls (VCF file)

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  1. Ion Proton Sequencing of paired Normal-tumor sample + All variant calls (VCF file) Sequencing Depth (Coverage file) OncoSeek Bioinformatics tool (Annotation, filtering and reporting tool) Low specificity variant filter Filters sequencing artifacts : High rate of occurrence or High population frequency Silent & non-exonic variants filter Filters synonymous, intronic and UTR variants Low sequencing depth filter & allelic fraction Filters variants with < 100X sequencing depth but retains them If reported in COSMIC database or >5% allelic fraction Germline variant filter Compares variants tumor and paired normal samples Filters variants with comparable variant frequency ( 10%) Somatic Mutations Confirmation by visual inspection of variants in IGV and absence in the paired normal Supplementary Figure 2

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