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Chemistry Shirts 2009: PowerPoint Presentation
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Chemistry Shirts 2009:

Chemistry Shirts 2009:

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Chemistry Shirts 2009:

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  1. Chemistry Shirts 2009: Peptide or periodic table designs also available. Order on Fridays pre-seminar in Chem. Library or from Dr. Raymond, CB 243

  2. Chemistry Seminar F 4/10 3:15 pm SL 130 Do try to attend. This guy is good!

  3. Office Hours this week: M 12-1 R 2-3

  4. Transcription

  5. Figure 31-1 The induction kinetics of b-galactosidase in E. coli. Page 1217

  6. Total RNA Pulse-chase MVA Fig. 26.3

  7. 3 Major Types of RNA: messenger transfer ribosomal Other small RNAs are involved in splicing.

  8. MVA Fig. 4.21

  9. (prok) Inh. initiation Euk inhibitor Blocks elongation MVA Fig. 26.4

  10. Table 31-1 Components of E. coli RNA Polymerase Holoenzyme. Page 1221

  11. RNAP IV!! Table 31-2 RNA Polymerase Subunitsa. Page 1232

  12. MVA Fig. 26.8

  13. Figure 31-11aX-Ray structure of Taq RNAP core enzyme. a subunits are yellow and green, b subunit is cyan, b¢ subunit is pink, w subunit is gray. What do you notice about this structure? Page 1224

  14. Figure 31-13bModel of the open (Rpo) complex of Taq RNAP with promoter-containing DNA showing the transcription bubble and the active site. Page 1225

  15. Figure 31-14 The two possible modes of RNA chain growth. Growth may occur (a) by the addition of nucleotides to the 3¢ end and (b) by the addition of nucleotides to the 5¢ end. Page 1226 How could you distinguish between these two possibilities?

  16. Cordycepin triphosphate

  17. Initiation

  18. MVA Fig. 26A. DNA footprinting (Nuclease protection assay)

  19. DNA Footprinting http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/F/ Footprinting.html

  20. Figure 31-10 The sense (nontemplate) strand sequences of selected E. coli promoters. Page 1223

  21. Figure 31-12a The sequence of a fork-junction promoter DNA fragment. Numbers are relative to the transcription start site, +1. Page 1225

  22. MVA Fig. 26.6

  23. Figure 31-15 RNA chain elongation by RNA polymerase. Page 1227

  24. Figure 31-16 An electron micrograph of three contiguous ribosomal genes from oocytes of the salamander Pleurodeles waltl undergoing transcription. Page 1228

  25. MVA Fig. 26.8

  26. RNA Backtracking MVA Fig. 26.10

  27. MVA Fig. 26.15

  28. MVA Fig. 26.16

  29. Figure 31-18 A hypothetical strong (efficient) E. coli terminator.