The Basics of Proposal Writing. An Introduction to Writing a Successful Grant Application. Introductory Comments: Is it about the money? Yes and No. Why write a grant proposal? Can you afford to support the projects that interest you from your own or your institution’s resources?
An Introduction to Writing a Successful Grant Application
in a positive manner to
improve ideas and
“The problems with this grant are legion, but if I go on to detail them I will have spent more time on the review than it appears the applicant did in his preparation of the proposal.”
Supporting Concepts & Data
Details of the Plan
Appendices (Use sparingly if permitted at all)
This is an important research project, which can be accomplished in timely fashion because the personnel, methods and equipment required for the successful completion of the research are already in place. The PI and co-investigators are well qualified to accomplish the goals of this application. The PI and co-investigators have worked closely together for several years, as evidenced by peer-reviewed papers which are relevant to the current proposal. The PI will be responsible for the organization of the research project and for the overall administration of the program. The PI has extensive experience in investigation of opioid-mediated signaling pathways and innate immunity. One Co-I has extensive experience in neuroscience and in glial biology. Another Co-I has extensive experience in cellular biology of neuroscience and another Co-I is experienced in researching signaling pathways. The research team will continue to interact via weekly lab meetings, email, phone and direct interaction.
cartoons to describe processes
Specific Aim 1. Elucidate the mechanism by which TLR2-mediated glial activation contributes to the development of opioid dependence and withdrawal
The working hypothesis for this aim is that TLR2-mediated glial activation plays a critical role in opioid dependence and withdrawal. The objective of this aim is to decipher the mechanisms by which TLR2 effects glial activation to produce opioid dependence and withdrawal thereby obtaining insights into the mechanisms causing opioid-induced glial activation. The rationalefor this aim is based on the following evidence. We know that TLR2 knockout mice develop attenuated morphine dependence (Fig. 5). We also know that chronic morphine administration significantly increases the expression of TLR2 in microglia (33). Importantly, we have shown that TLR2 is required for the glial cell activation following opioid dependence (Fig. 7). In addition, TLR2 deficiency in mice significantly reduces the levels of proinflammatory cytokines following chronic morphine administration (Fig. 8). Moreover, recent evidence reveals that TLR2 can function as a cell death receptor (12). However, we do not know the mechanism by which TLR2-mediated glial cell activation contributes to the development of opioid dependence and withdrawal. The experiments in this aim will focus on this issue. The strategy of this aim is to block TLR2 in mice in order to determine the contribution of TLR2-mediated glial activation in producing opioid dependence and withdrawal.
Intellectual Merit. Scanning electron microscopy is an important investigative tool in the life and physical sciences. There are three major research programs and at least four additional research programs at East Tennessee State University (ETSU) that currently require high resolution SEM and/or EDS. Further, ETSU is beginning a Department of Geosciences in 2009 which will increase demand on the SEM. The expanding use of the existing scanning electron microscopes in research and teaching by several units and the age of the existing SEMs demands improved infrastructure in this area in order to provide adequate support for research and teaching in several departments at ETSU.
The purpose of this grant is to specifically upgrade ETSU’s SEM capabilities. The existing Zeiss DSM 940 and the Hitachi S-340, because of their age (greater than 25 years), condition, and the availability of parts have become increasingly difficult to maintain, and the resolution and quality of the images these instruments produce is not adequate for high quality research. Further, there is a critical need to add analytical capabilities to our SEM (i.e., EDS). In an evaluation of our EM facility users have unanimously viewed the acquisition of a high resolution SEM as the most immediate and critical research need.
Broader Impacts. The number faculty members using SEM has increased at ETSU during the past five years and there has been a concomitant increase in undergraduate and graduate student research projects necessitating more frequent offering of formal instruction in electron microscopy to prepare students to conduct independent undergraduate and graduate research projects. Investigators are more frequently using electron microscopic methods and analytical techniques in undergraduate and graduate classes (e.g. Mineralogy, Sedimentation - Stratigraphy, Structural Geology, Invertebrate Paleontology and Vertebrate Paleontology). SEM is used in these courses for, among other things, identification of mineral species, determination of the shape and form of minerals, and sedimentary grain analysis but because of age of the current instruments students are not being trained in the use of modern equipment and analytical techniques which will be corrected with the SEM requested in this proposal.
Without question an improved SEM facility will strengthen existing research programs, attract new research programs, and allow training of students at all levels, including K-12, in the use of SEM. The new SEM will significantly facilitate research programs at ETSU in biological sciences, paleontology, geology, anatomy and cell biology, microbiology and physiology. Further, it will also serve faculty and students at King College and Virginia Intermount College, liberal arts colleges located about 25 miles from ETSU. It will increase research capability at King in the area of phycology particularly in the study of algal diversity and taxonomy. Additionally students taking courses in Microbiology, Histology, and Photosynthetic Life at King will use the requested SEM.
The ETSU and General Shale Brick Natural History Museum provides an ideal venue to introduce students in grades K-12 to “hands on” education in research methods in the life and physical sciences. The purchase of the requested equipment will significantly contribute to the development and implementation of primary and secondary school programs through the Museum of Natural history, as well as exposing the adult general public to the use of this technology in research.