2. Problema medico. Patients suffering from Cooley\'s anemia (Thalassemia major) need frequent blood transfusions. Transfused red cell iron accumulates in tissues and organs with toxic effects . The most common method for iron evaluation is the liver biopsy. Assessment of body-iron accumulation is essential for managing therapy of iron-chelating diseases characterized by iron overload such as thalassemia, hereditary hemochromatosis, and other forms of severe anemia. .