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Lysmikroskopi

Lysmikroskopi. MENA3100,OBK, 01.02.11. Lysmikroskop. Optisk mikroskop. Forstørrelse (magnification) Oppløsningsevne (resolution) Kontrast. Vi ønsker å se flere detaljer. Simple microscope. Lupe, forstørrelsesglass. The simple microscope. Antony van Leeuwenhoek. (1632−1723).

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Lysmikroskopi

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  1. Lysmikroskopi MENA3100,OBK, 01.02.11

  2. Lysmikroskop Optisk mikroskop Forstørrelse (magnification) Oppløsningsevne (resolution) Kontrast Vi ønsker å se flere detaljer

  3. Simple microscope Lupe, forstørrelsesglass

  4. The simple microscope Antony van Leeuwenhoek (1632−1723)

  5. Det sammensatte mikroskop The compound microscope ca. 1595 Hans Janssen Robert Hooke (1635−1703) Micrographia, 1664

  6. Don’t get too hang up in the ray tracing

  7. Stereomikroskop Stereo microscope

  8. Mikroskop for gjennomfallende lys Compound microscope for transmitted light

  9. Mikroskop for reflektert lys Inverted compound microscope for reflected light

  10. www.microscopy-analysis.com/files/ jwiley_microscopy/2006_Sept_Hammond.pdf

  11. Illuminating system Specimen Condenser lens Aperture diaphragm Field diaphragm Filament

  12. Illuminating system Specimen Condenser lens Aperture diaphragm Köhler illumination Uniform specimen illumination Field diaphragm Controll illuminated area Field diaphragm Aperture diaphragm Controll angle of the cone of light Filament

  13. Imaging system Eyepiece Okular Tube lens Objective Specimen

  14. Primary image

  15. Refraction

  16. Kromatisk abberation

  17. Spherical abberation

  18. Corrections The objective

  19. The limiting factor: Diffraction

  20. Resolution R = 0,61l/NA Rayleigh criterion (Raleigh in Brandon and Kaplan)

  21. Abbe

  22. Numerical aperture (NA) NA = nsina Resolution R = 0,61l/NA

  23. Oil immersion

  24. Fouriertransformasjon

  25. More is needed than just the objective Condenser system Eyepice Ocular (okular)

  26. Optical anisotropy Calcite

  27. Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices Lower symmetry: Optically anisotropic, three refractive indices

  28. Optically active crystals in the microscope

  29. Optically active crystals in the microscope

  30. Michel-Levy chart

  31. Remember that geologists often call their book on microscopy on thin sections for optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi). Some web sites: http://www.olympusmicro.com/primer/ http://www.microscopyu.com/ http://www.micro.magnet.fsu.edu/ http://www.micro.magnet.fsu.edu/primer/pdfs/basicsandbeyond.pdf maintenance: http://www.zeiss.de/C1256D18002CC306/0/1205E8CBD68054EAC125701500404705/$file/50-1-0011_e.pdf

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