What was the purpose of putting 1.5ml of Gelatin solution into your 6 well trays?

1. What was the purpose of putting 1.5ml of Gelatin solution into your 6 well trays? • Kill any contaminants • Create a hydrophobic surface to grow MEFs • Create a hydrophilic surface to grow MEFs • To dilute MEFs after adding them in D10 medium

2. Where are ES cells taken from originally? • Fetus • Adult organs • Embryo • Mice

3. Cells that are pluripotent are special in that they can • become any cells in the body • become a few different cells in the body • only double themselves a few times before dying • Kill cancer cells before they metastasize

4. After thawing your MEF vial in the water bath, you performed a “wash” using D10 solution. What was the purpose of the wash? • To remove the DMSO used to freeze the cells, but which is also toxic to the cells • To make sure the cells were properly dissolved in the D10 solution • To separate the cells into 2 different centrifuge tubes • To ensure the cells had completely thawed prior to plating in the wells

5. Mitomyocin C is used on the MEF cells to prevent them from growing. MitoC does this by • Inactivates the ribsosomes • Cutting the DNA into pieces • Denaturing Mitotic proteins • Cross-linking DNA strands

6. How much of a 1mg/ml stock solution should be used to make a 10 microgram/ml in a working solution of 50ml total? • 1 ml • 10 microliter • 50 microliter • 0.5 ml