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Dynamics of Bcl-2 Expression in Mia PaCa-2 and AsPC-1 Cells

This study investigates Bcl-2 expression in Mia PaCa-2 and AsPC-1 cells, determining optimal harvesting time and kinetics post-transfection. Caspase activation was monitored every 12 hours, showing a consistent response. Bcl-2 was undetectable in AsPC-1 cells by Western blot.

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Dynamics of Bcl-2 Expression in Mia PaCa-2 and AsPC-1 Cells

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  1. B A kDa Bcl-2 GAPDH 29 Mia PaCa-2 AsPC-1 AsPC-1 Supporting Information 1. Supporting Information 1: In AsPC-1 cells Bcl-2 expression was not detectable using western blot (A). We determined the optimal time point for harvesting by activation of caspase 3 and 7. The groups displayed a similar kinetic response to transfection. The dynamic measurement was conducted every 12 hours after transfection. All Caspase assays were done in duplicate (B).

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