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Plant Respiration and Alternative Pathways in Ecology

Dive into the complexities of plant respiration, from autotrophic to alternative pathways, and explore the role of fluorescence in estimating mesophyll conductance. Discover how respiration models influence plant growth and the regulatory mechanisms involved in this essential process.

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Plant Respiration and Alternative Pathways in Ecology

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  1. Feb. 3rd, 2011B4730/5730Plant Physiological Ecology Respiration I

  2. Fs is steady state Fluorescence (F’) Closed circle F’m amb CO2 Closed triangle Fs amb CO2 Open circle F’m elev CO2 Open triangle Fs elev CO2

  3. Leipner et al Env. Exp. Bot. 1999 Maize

  4. Mesophyll conductance • Flux of CO2 to stroma (Cc) limited by gs, gm and gb • Recent work has shown gm to be variable and important • Low gm reduces CC underestimate VCmax • Cc=Ci-A/gm • Estimate gm with curve fitting • Estime gm with fluorescence and A

  5. Hedera helix; Pons et al J Exp Bot 2009

  6. Many species; Sc= surface area of chloroplasts exposed to intercellular airspace; Evans et al J Exp Bot 2009

  7. Limitations to the Farquhar/Fluorescence model of Photosynthesis • No gene expression controls • Connection to hormones, whole plant controls • Longer time scales • Stomatal physiology almost a black box • Scale of leaf patchiness violates homogeneity • Light dynamics problematic • Respiration models are crude

  8. Autotrophic Respiration • Can not describe plant growth from photosynthesis only • High N requirements for enzymes of both • Total Respiration is the sum of • Maintenance • Growth • Transport • All eukaryotes with mitochondria have cytochrome respiratory pathway • Inhibited by cyanide • Plants have cyanide resistant pathway that uses AOX • animal regulation before glycolysis, plants during

  9. Autotrophic Respiration • Respiration can be regulated by energy demand or substrate supply • Photosynthesis and respiration connected through chemiosmosis (AOX) • Ratio of CO2 to O2 (respiratory quotient) varies with tissue type and substrates used • <1 more reduced substrates • >1 more reduced products

  10. Alternative Respiration Pathway • Alternative Respiration pathway (CN resistant) • Respiration production exceeds demand, reactive oxygen species (ROS) activated • Uses alternative oxidase (AOX) instead of cytochrome electron transport chain • Generates heat • Various hypotheses about regulation and purpose • Uncoupling proteins (UCP) connect to photosynthesis • Uncoupling proteins relieve excess pH gradient in mitochondria • Photorespiration inhibited by loss of UCP, ROS problems

  11. Kruse et al New Phyt 2011

  12. Philodendron bipinnatifidum Miller et al New Phyt 2010

  13. Alternative respiration white; Gray total respiration

  14. Cucumis sativus; Juszcuk et al Phys Plant 2007 MSC16 mutant with lowered respiration/growth rates encoded by mitochondrial genome

  15. Arabidopsis thaliana; Sweetlove et al PNAS 06 ucp1 mutant without uncoupling protein

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