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ENZYME CLASSIFICATION EXERCISE

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ENZYME CLASSIFICATION EXERCISE. (1) GLUCOSE + ATP  GLUCOSE-6-PHOSPHATE + ADP + H + (2) CH 3 CH 2 OH + NAD +  (CH3)CHO + NADH + H + (3) ATP + H 2 O  ADP + P i (4) CH 3 (CO)COO - + H +  CH 3 CHO + CO 2 (PYRUVATE) (ACETALDEHYDE)

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slide1
ENZYME CLASSIFICATION EXERCISE

(1) GLUCOSE + ATP  GLUCOSE-6-PHOSPHATE + ADP + H+

(2) CH3CH2OH + NAD+ (CH3)CHO + NADH + H+

(3) ATP + H2O  ADP + Pi

(4) CH3(CO)COO- + H+  CH3CHO + CO2

(PYRUVATE) (ACETALDEHYDE)

(5) CH3(CO)COO- + ATP + CO2  ADP + Pi + -OOC-CH2 (C0) COO-

(OXALOACETATE)

-OOC H H H

| | | |

(6) C = C  C = C

| | | |

H COO- -OOC COO-

slide2
ENZYME CLASSIFICATION EXERCISE

(7) OXALOACETATE + GTP  PHOSPHOENOLPYRUVATE + GDP + CO2

(8) ASP + -KETOGLUTARATE  OXALOACETATE + GLU

(9) 3-PHOSPHOGLYCERATE  2-PHOSPHOGLYCERATE

(10) -OOC-CH2-CH2-COO- + FAD  -OOC-CH=CH-COO- + FADH2

slide3
ENZYME CLASSIFICATIONANSWERS TO IN-CLASS EXERCISE
  • (1) TRANSFERASE (HEXOKINASE)
  • (2) OXIDOREDUCTASE (ALCOHOL DEHYDROGENASE)
  • (3) HYDROLASE (ATPase)
  • (4) LYASE (PYRUVATE DECARBOXYLASE)
  • (5) LIGASE (PYRUVATE CARBOXYLASE)
  • (6) ISOMERASE (MALEATE ISOMERASE)
  • (7) HYDROLASE (PHOSPHOENOLPYRUVATE CARBOXYKINASE)
  • (8) TRANSFERASE (AMINOTRANSFERASE)
  • (9) PHOSPHOGLYCERATE MUTASE
  • (10) OXIDOREDUCTASE (SUCCINATE DEHYDROGENASE)
slide4
OXIDOREDUCTASES
  • CATALYZE REDOX REACTIONS
  • SOME COMMON TYPES:
    • REDUCTASES
    • DEHYDROGENASES
    • OXIDASES
    • OXYGENASES
  • LOOK FOR INVOLVEMENT OF COENZYMES
    • NAD+ , NADP+, FAD, FMN
slide5
TRANSFERASES
  • TRANSFER OF FUNCTIONAL GROUPS
  • SOME EXAMPLES:
    • TRANSFERASES
    • PHOSPHORYLASES
      • TRANSFER OF Pi OR PPi
    • KINASES
      • A PHOSPHATE TRANSFER
      • UTILIZE OR GENERATE ATP
slide6
HYDROLASES
  • HYDROLYSIS REACTIONS
  • WATER MOLECULES USED TO BREAK BONDS
  • EXAMPLES:
    • PHOSPHATASES
    • PEPTIDASES
    • ATPase, GTPase
slide7
LYASES
  • CATALYZES REACTIONS THAT
    • GENERATE A DOUBLE BOND
    • ADDS A SUBSTRATE MOLECULE TO DOUBLE BOND OF A SECOND SUBSTRATE
  • EXAMPLES:
    • DECARBOXYLASES
    • DEHYDRATASES
    • ALDOLASE
slide8
ISOMERASES
  • CONVERSION OF ONE ISOMERIC FORM INTO ANOTHER
  • EXAMPLES:
    • ISOMERASES
    • EPIMERASES
    • RACEMASES
    • MUTASES
slide9
LIGASES
  • TWO MOLECULES ARE JOINED
  • ANABOLIC REACTIONS
  • REQUIRE NUCLEOTIDES (ATP, GTP) TO DRIVE THEM
    • A PYROPHOSPHATE BOND MUST BE BROKEN
  • EXAMPLES:
    • CARBOXYLASE
    • SYNTHETASES
  • DON’T CONFUSE SYNTHETASES WITH SYNTHASES
ongoing exercise
ONGOING EXERCISE
  • USING THE PRECEEDING PRINCIPLES OF ENZYME CLASSIFICATION, MAKE SURE THAT YOU CAN IDENTIFY THE CLASS OF ENZYME FOR EACH ENZYME THAT WE STUDY AND RATIONALIZE WHY IT FITS INTO THAT PARTICULAR CLASS
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