1 / 9

PCR = DNA ?

PCR = DNA ?. LB 145 January 27, 2011. Does PCR happen inside your body? (How do you raise your temp to 95°C?) PCR was invented in the last 30 years. It exploits our knowledge of another process called ??. Through the process of DNA Replication. Why?.

Download Presentation

PCR = DNA ?

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. PCR = DNA ? LB 145 January 27, 2011

  2. Does PCR happen inside your body? (How do you raise your temp to 95°C?) • PCR was invented in the last 30 years. It exploits our knowledge of another process called ??...

  3. Through the process of DNA Replication

  4. Why? • Each cell must have a way to copy its entire genome • Cell Division • Damage Control Where does this take place?

  5. Check this out • http://www.youtube.com/watch?v=teV62zrm2P0&feature=related • http://www.youtube.com/watch?v=AGUuX4PGlCc

  6. PCR vs. DNA Replication • Similarities? • Copy DNA • Move 5’-3’ via a polymerase enzyme • require primers

  7. PCR vs. DNA Replication • Differences? • Constant temperature (body temp is 37C) • Enzymes are the workhorses • All 3 stages happen simultaneously • Multiple enzymes vs. Taq Polymerase • Leading/Lagging Strand • RNA primers vs DNA primers

  8. So, what are we doing again? • PCR is selective DNA replication, it exploits our knowledge of the DNA rep. • Instead of using difficult (and expensive) enzymes in the body we used more controllable, predictable PCR machines.

More Related